Abstract
The use of chemical and UV-induced mutageneses allowed us to increase the biosynthetic activity of the strain capable of producing new antistaphylococcal antibiotic, batumin. The strain ofPseudomonas batumici N17 producing 87–100 mg batumin per liter culture liquid was selected. Its activity was 3.5-5 times higher than the activity of the most potent natural strain.P. batumici N17 was shown to be stable in relation to the synthesis of batumin.
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Kiprianova, E.A., Boiko, O.I., Rabinovich, A.O., and Aizenman, B. Yu.,Mikrob. Zh., 1974, vol. 36, no. 6, pp. 781–783.
Esipov, S.E. and Kiprianova, E.A., Abstracts of Papers, (5th Int. Conf. on Chemical Synthesis of Antibiotics and Related Microbial Products), Budapest, 1996, p. 14.
Smirnova, V.V., Churkina, L.N., Kravets, A.N., and Garagalya, A.D.,Antibiot. i khimioterapiya, 1993, vol. 38, no. 4-5, pp. 3–5.
Miller, J.,Experiments in Molecular Genetics, New York: Cold Spring Harbor Laboratory, 1972. Translated under the titleEksperimenty vmolekulyarnoi genetike, Moscow: Mir, 1976.
Stolyar, S.M., Romanovskaya, V.A., Budkova, E.N., and Malashenko, Yu.R.,Mikrbiol. Zh. (Ukraina), 1993, vol. 55, no. 1, pp. 47–53.
Maniatis, T., Fritsch, E., and Sambrook, J.,Molecular Cloning. A Laboratory Manual, New York: Cold Spring Harbor Laboratory, 1982. Translated under the titleMolekulyarnoe klonirovanie, Moscow: Mir, 1984.
Vikhanskii, Yu.D., Yarulin, V.R., Esipova, V.V., Bartoshevich, Yu.E., Zhdanovich, Yu.V., and Nasonova, L.I.,Antibiotiki i khimioterapiya, 1992, vol. 37, no. 6, pp. 5–7.
Mehta, B.J. and Cerda-Olmedo, E.,Appl. Microbiol. Biotechnol, 1995, no. 6, pp. 836–838.
Drake, J.,Molecular Basis of Mutation, San-Francisco: Holden-Day, 1970, pp. 161–176.
Murphy, H.S., Palejwala, V.A., Rahman, M.S., Dunman, P.M., Wang, G., and Humayun, M.Z.,J. Bacteriol., 1996, vol. 178, no. 23, pp. 6651–6657.
Modrich, P.,Annu. Rev. Genet., 1991, vol. 25, pp. 229–253.
Murli, S. and Walker, G.C.,Curr. Opin. Genet. Dev., 1993, vol. 3, no. 5, pp. 719–725.
Lavrent’eva, G.I., Zhukova, R.A., Demchenko, L.N., and Kotenko, T.V.,Antibiotiki, 1973, vol. 18, no. 8, p. 692.
Erokhina, L.I.,Genetika, 1968, vol. 4, no. 11, p. 101.
Hadden, C., Foote, R., and Mitra, S.,J. Bacterioi, 1983, vol. 153, no. 2, pp. 756–762.
Wang, G.V., Palejwala, V.A., Dunman, P.M., Aviv, D.H., Murphy, H.S., Rahman, M.S., and Humayun, M.Z.,Genetics, 1995, vol. 141, no. 3, pp. 813–823.
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Smirnov, V.V., Churkina, L.N., Perepnikhatka, V.I. et al. Obtaining a high-activity strain capable of producing the antistaphylococcal antibiotic batumin. Appl Biochem Microbiol 36, 46–49 (2000). https://doi.org/10.1007/BF02738134
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DOI: https://doi.org/10.1007/BF02738134