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Separation of phospholipids from soybean by NP-HPLC with ELSD

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Abstract

Phospholipids of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) from lecithin were separated on gradient steps by NP-HPLC with ELSD. The ternary mobile phases of hexane, IPA, and water were used, and the optimum mobile phase conditions were experimentally determined. The calibration curves of phospholipids were constructed to analyze quantitatively the amounts of PE and PC. The responses of PE by ELSD showed linearity with the injected amount range but a logarithmic relationship of PC was observed. By quantitative analysis, 14.2% of PE and 36.6% (w/w) of PC were contained in the sample of lecithin. Finally, in the separation of phospholipids, the detection by ELSD was better than that by UV.

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Authors and Affiliations

  1. Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University, 253 Yonghyun-Dong, Nam-Ku, 402-751, Incheon, Korea

    Duk Hui Kang, Sueng Ki Lee & Kyung Ho Row

Authors
  1. Duk Hui Kang
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  2. Sueng Ki Lee
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  3. Kyung Ho Row
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Corresponding author

Correspondence to Kyung Ho Row.

Additional information

This paper is dedicated to Dr. Youn Yong Lee on the occasion of his retirement from Korea Institute of Science and Technology.

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Kang, D.H., Lee, S.K. & Row, K.H. Separation of phospholipids from soybean by NP-HPLC with ELSD. Korean J. Chem. Eng. 19, 818–820 (2002). https://doi.org/10.1007/BF02706974

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  • DOI: https://doi.org/10.1007/BF02706974

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