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Pleurotus sajor-caju HSP100 complements a thermotolerance defect inhsp104 mutantSaccharomyces cerevisiae

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An Erratum to this article was published on 01 September 2006

Abstract

A putativeHsp100 gene was cloned from the fungusPleurotus sajor-caju. mRNA expression studies demonstrated that this gene (designatedPsHsp100) is highly induced by high temperature, induced less strongly by exposure to ethanol, and not induced by drought or salinity. Heat shock induction is detectable at 37‡C and reaches a maximum level at 42‡C.PsHsp100 mRNA levels sharply increased within 15 min of exposure to high temperature, and reached a maximum expression level at 2 h that was maintained for several hours. These results indicate that PsHsp100 could work at an early step in thermotolerance. To examine its function,PsHsp100 was transformed into a temperature-sensitivehsp104 deletion mutantSaccharomyces cerevisiae strain to test the hypothesis that PsHSP100 is an protein that functions in thermotolerance. Overexpression of PsHSP100 complemented the thermotolerance defect of thehsp104 mutant yeast, allowing them survive even at 50‡C for 4 h. These results indicate that PsHSP100 protein is functional as an HSP100 in yeast and could play an important role in thermotolerance inP. sajor-caju.

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Abbreviations

HSPs:

Heat shock proteins

MCM:

mushroom complete medium

ORF:

open reading frame

PCR:

polymerase chain reaction

RT:

reverse transcription

UTR:

untraslated region

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Authors and Affiliations

  1. National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707, Suwon, Korea

    Jin-Ohk Lee, Mi-Jeong Jeong, Tack-Ryun Kwon, Seung-Kon Lee, Myung-Ok Byun, Ill-Min Chung & Soo-Chul Park

  2. Department of Agronomy, Konkuk University, 143-701, Seoul, Korea

    Ill-Min Chung

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  1. Jin-Ohk Lee
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  2. Mi-Jeong Jeong
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  3. Tack-Ryun Kwon
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  4. Seung-Kon Lee
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  6. Ill-Min Chung
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  7. Soo-Chul Park
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Correspondence to Ill-Min Chung or Soo-Chul Park.

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Lee, JO., Jeong, MJ., Kwon, TR. et al. Pleurotus sajor-caju HSP100 complements a thermotolerance defect inhsp104 mutantSaccharomyces cerevisiae . J Biosci 31, 223–233 (2006). https://doi.org/10.1007/BF02703915

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