Skip to main content
SpringerLink
Log in

Behavior of plastid nucleoids during male gametogenesis inPlumbago auriculata

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

We characterized the behavior of plastid (pt) and mitochondrial (mt) nucleoids during malegametogenesis inPlumbago auriculata in three dimensions. The behavior of pt-nucleoids and mt-nucleoids differed throughout male gametogenesis.Pt-nucleoids were distibuted in a characteristic manner in three stages: in the early microspore, pt-nucleoids assemble around cell nucleus: in the mid-generative cell,pt-nucleoids gather at the internal side of the pollen: in the late-generative cell, pt-nucleoids aggregation turns its pole to the external side of the pollen. We also studied organelle nucleoids in the egg and the central cell by a method in which semi-thick sections of resin-embedded anthers and ovaries were observed by confocal laser scanning microscopy. The number of pt-nucleoids in the sperm cell did not differ significantly from that the behavior of DNA-containing organelles is regulated strictly during male gametogenes inP. auriculata, and that a biparental inheritance of plastids in the Plumbago embryo is more favored than was previously thought.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DAPI:

4′,6-diamidino-2-phenylindole

DiOC6 :

3,3′-dihexyloxacarbocyanine iodide

Sua :

sperm cell unassociated with vegetative nucleus

Svn :

sperm cell associated with vegetative nucleus

References

  • Kuroiwa H, Ohta T, Kuroiwa T (1996) Studies on the development and three-dimensional reconstruction of giant mitochondria and their nuclei in egg cells ofPelargonium zonale Ait. Protoplasma 192: 235–244

    Article  Google Scholar 

  • Kuroiwa T, Fujie M, Mita T, Kuroiwa H (1991) Application of embedding of samples in Technovit 7100 resin to observations of small amounts of DNA in the cellular organelles associated with cytoplasmic inheritance. Appl Fluor Tech 3: 23–25

    Google Scholar 

  • — Kuroiwa H, Fujie M (1992) Studies on the behavior of mitochon-drial DNA: synthesis of mitochondrial DNA occurs actively in a specific region just above the quiescent center in the root meri-stem ofPelargonium zonale. J Cell Sci 101:483–493

    CAS  Google Scholar 

  • Nagata N, Saito C, Sakai A, Kuroiwa H, Kuroiwa T (1999) The selec-tive increase or decrease of organellar DNA in generative cells just after pollen mitosis one controls cytoplasmic inheritance. Planta 209: 53–65

    Article  PubMed  CAS  Google Scholar 

  • Russell SD (1980) Participation of male cytoplasm during gamete fusion in an angiosperm,Plumbago zeylanica. Science 210: 200–201

    Article  PubMed  Google Scholar 

  • — (1984) Ultrastructure of the sperm ofPlumbago zeylanica 2: quantitative cytology and 3-dimensional organization. Planta 162: 385–391

    Article  Google Scholar 

  • — (1985) Preferential fertilization inPlumbago zeylanica: ultra-structural evidence for gamete-level recognition in an angiosperm. Proc Natl Acad Sci USA 82: 6129–6132

    Article  PubMed  CAS  Google Scholar 

  • — (1987) Quantitative cytology of the egg and central cell ofPlumbago zeylanica and its impact on cytoplasmic inheritance patterns. Theor Appl Genet 74: 693–699

    Article  Google Scholar 

  • — Cass DD (1981) Ultrastructure of the sperm ofPlumbago zeylanica 1: cytology and association with the vegetative nucleus. Protoplasma 107: 85–107

    Article  Google Scholar 

  • Strout GW, Stramski AK, Mislan TW, Thompson RA, Schoemann LM (1996) Microgametogenesis inPlumbago zeylanica (Plumbaginaceae) 1: descriptive cytology and three-dimensional organization. Am J Bot 83:1435–1453

    Article  Google Scholar 

  • Sakai A, Kawano S, Kuroiwa T (1992) Conversion of proplastids to amyloplasts in tobacco cultured cells is accompanied by changes in the transcriptional activities of plastid genes. Plant Physiol 100: 1062–1066

    Article  PubMed  CAS  Google Scholar 

  • Sodmergen, Chen GH, Hu ZM, Guo FL, Guan XL (1995) Male gametophyte development inPlumbago zeylanica: cytoplasm localization and cell determination in the early generative cells. Protoplasma 186: 79–86

    Article  Google Scholar 

  • Saito C, Fujie M, Sakai A, Kuroiwa H, Kuroiwa T (1997) Changes in the extent of the condensation of nuclear chromatin and the localization of RNA during pollen development inNicotiana tabacum. Cytologia 62:121–132

    CAS  Google Scholar 

  • ——— (1998) Detection and quantification of rRNA by high-resolution in situ hybridization in pollen grains. J Plant Res 111:45–52

    Article  CAS  Google Scholar 

  • — Hayashi M, Sakai A, Fujie M, Kuroiwa H, Kuroiwa T (1999) Improved sensitivity for high-resolution in situ hybridization using resin extraction of methyl methacrylate embedded mater-ial. Biotech Histochem 74: 40–48

    PubMed  CAS  Google Scholar 

  • Tanaka I (1991) Microtubule-determined plastid distribution during microsporogenesis inLilium longiflorum. J Cell Sci 99:21–31

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. A. Sakai

    Present address: Department of Biological Science, Faculty of Science, Nara Women’s University, Nara, Japan

Authors and Affiliations

  1. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo

    C. Saito, N. Nagata, A. Sakai, H. Kuroiwa & T. Kuroiwa

  2. Institute of Physical and Chemical Research, Wako, Saitama, Japan

    C. Saito, N. Nagata & A. Sakai

Authors
  1. C. Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Nagata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Sakai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Kuroiwa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Kuroiwa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saito, C., Nagata, N., Sakai, A. et al. Behavior of plastid nucleoids during male gametogenesis inPlumbago auriculata . Protoplasma 216, 143–154 (2001). https://doi.org/10.1007/BF02673866

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02673866

Keywords

Search

Navigation