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Sterol composition of normal and habituated sugarbeet callus (Beta vulgaris L.Altissima)

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Summary

A detailed qualitative and quantitative analysis of the sterol content of normal (auxin and cytokinin requiring) and habituated (auxin and cytokinin independent) sugarbeet callus (Beta vulgaris L.altissima) was made using mass spectrometry with gas chromatography. The total sterol content of the two lines did not differ significantly. Δ7-Sterols were the most important class of sterols in the two sugarbeet callus lines, as in allChenopodiaceae. Elevated levels of Δ8-sterols were found in the habituated callus. These sterols are considered to be badly integrated in the membrane of eucaryotic cells. A partial blocking of Δ8-Δ7-isomerase is hypothesized in the habituated cell line.

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Authors and Affiliations

  1. Enzymologie Cellulaire et Moléculaire, Institut de Botanique, IBMP, 28 rue Goethe, F-67083, Strasbourg, France

    Paulette Schmitt

  2. Hormonologie fondamentale et appliquée, Institut de Botanique B22, Université de Liège, Sart Tilman, B-4000, Liège, Belgique

    Thomas Gaspar

  3. Biochimie Végétale, UFR Sciences et Techniques, BP 452, F-29275, Brest cedex, France

    Daniel Hagège

Authors
  1. Paulette Schmitt
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  2. Thomas Gaspar
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  3. Daniel Hagège
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Schmitt, P., Gaspar, T. & Hagège, D. Sterol composition of normal and habituated sugarbeet callus (Beta vulgaris L.Altissima). In Vitro Cell Dev Biol - Plant 30, 1–3 (1994). https://doi.org/10.1007/BF02632111

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  • DOI: https://doi.org/10.1007/BF02632111

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