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Establishment and characterization of bovine mammary epithelial cell lines

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An Erratum to this article was published on 01 January 1993

Summary

One bovine mammary epithelial cell clone, designated PS-BME-C1, and two bovine mammary epithelial cell lines, designated PS-BME-L6 and PS-BME-L7, were derived from mammary tissue of a pregnant (270 day) Holstein cow. The cells exhibit the distinctive morphologic characteristics of mammary epithelial cells and express the milk fat globule membrane protein, PAS-III. They form domes when cultured on plastic substrata and acinilike aggregates when cultured on a collagen matrix. These cells are capable of synthesizing and secretingα-lactalbumin andα-s1-casein when cultured on a collagen matrix in the presence of insulin, cortisol, and prolactin. The cells have a near-normal diploid number and do not grow in suspension culture. When transplanted to the cleared mammary fat pads of female athymic nude mice, the cells readily proliferate forming noninvasive palpable spherical cellular masses within 8 wk after inoculation. The cells may become a useful tool to study the regulation of ruminant mammary epithelial cell growth and differentation.

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Authors and Affiliations

  1. Department of Dairy and Animal Science, The Pennsylvania State University, 302 Henning Building, 16802, University Park, Pennsylvania

    C. Anne Gibson, Juan R. Vega & Craig R. Baumrucker

  2. Department of Pharmacology and Toxicology, Michigan State University, 48824, East Lansing, Michigan

    Cori S. Oakley & Clifford W. Welsch

Authors
  1. C. Anne Gibson
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  2. Juan R. Vega
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  3. Craig R. Baumrucker
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  4. Cori S. Oakley
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  5. Clifford W. Welsch
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Additional information

This work was supported by the Pennsylvania State University Experiment Station.

The PS-BME cells are the property of The Pennsylvania Research Corporation. Scientists interested in obtaining the PS-BME clone or cell lines for their research may request them from the corresponding author.

An erratum to this article is available at http://dx.doi.org/10.1007/BF02634376.

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Gibson, C.A., Vega, J.R., Baumrucker, C.R. et al. Establishment and characterization of bovine mammary epithelial cell lines. In Vitro Cell Dev Biol - Animal 27, 585–594 (1991). https://doi.org/10.1007/BF02631290

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  • DOI: https://doi.org/10.1007/BF02631290

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