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Growth factor binding and bioactivity in human kidney epithelial cell cultures

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Summary

Insulinlike growth factors (IGF) and epidermal growth factor (EGF) are produced in renal tissue, as are specific receptors for these hormones. To evaluate the significance of these observations to regulation of renal tubular cell proliferation, we have examined the interaction of IGF and EGF with cultured human proximal tubular epithelial cells (HPT). HPT cells showed specific binding of IGF-1, insulin, and EGF. IGF-1 binding was inhibited by antibody to the type 1 IGF receptor (α-IR3). Insulin receptors and type 1 IGF receptors were identified by bifunctional cross-linking. IGF-1, insulin, and EGF stimulated [3H]thymidine incorporation by 77, 73, and 87%, respectively. Haft maximal stimulation by IGF-1, insulin, and EGF was produced with 4×10−9 M, 2.5×10−8 M, and 8×10−10 M concentrations of these hormones. α-IR3 inhibited stimulation of thymidine incorporation by IGF-1 and insulin but had no effect in EGF-stimulated thymidine incorporation. EGF and high concentrations of insulin both stimulated cell proliferation by 83 and 79%, respectively. These data are consistent with regulation of tubular epithelial proliferation by IGF-1, insulin, and EGF and suggest that the mitogenic activity of both insulin and IGF-1 is mediated by the type 1 IGF receptor.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, South Carolina

    Ted Gansler, Wei-Chao Hsu, T. Stokes Gramling, Ned Blocker, Sadie Green, A. Julian Garvin & Donald A. Sens

  2. Department of Medicine, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, South Carolina

    Katherine A. Robinson & Maria G. Buse

  3. Department of Neurology, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, South Carolina

    Linda Roy

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  1. Ted Gansler
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  2. Wei-Chao Hsu
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  3. T. Stokes Gramling
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  4. Katherine A. Robinson
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  7. Linda Roy
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  9. A. Julian Garvin
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  10. Donald A. Sens
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Additional information

Supported by grants CA37887 and DK32889 from the National Institutes of Health, Bethesda, MD, and by a Medical University of South Carolina institutional grant.

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Gansler, T., Hsu, WC., Gramling, T.S. et al. Growth factor binding and bioactivity in human kidney epithelial cell cultures. In Vitro Cell Dev Biol 26, 285–290 (1990). https://doi.org/10.1007/BF02624459

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  • DOI: https://doi.org/10.1007/BF02624459

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