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Improved isolation of dihaploidsolanum tubersoum protoplasts and the production of somatic hybrids between dihaploidS. tuberosum andS. brevidens

  • Plant Cellular and Developmental Biology
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Summary

A modified protoplast isolation technique, applicable to a range of dihaploidSolanum tuberosum genotypes, has been developed. A combination of high calcium and high pH was used to fuse mesophyl protoplasts of dihaploidS. tuberosum (PDH40) and the diploid wild speciesS. brevidens. Large numbers of colonies were obtained after fusion and putative hybrids selected on the basis of phenotype from regenerated shoots. From these, 11 somatic hybrid plants have been identified by their isoenzyme patterns and morphologic characteristics. Four of these hybrids had the expected chromosome number of 48. The approach of mass culture after fusion followed by selection of hybrids from regenerated shoots and the application of somatic hybridization to potato breeding are discussed.

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Authors and Affiliations

  1. Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK

    N. Fish, A. Karp & M. G. K. Jones

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  1. N. Fish
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  2. A. Karp
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  3. M. G. K. Jones
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Fish, N., Karp, A. & Jones, M.G.K. Improved isolation of dihaploidsolanum tubersoum protoplasts and the production of somatic hybrids between dihaploidS. tuberosum andS. brevidens . In Vitro Cell Dev Biol 23, 575–580 (1987). https://doi.org/10.1007/BF02620975

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  • DOI: https://doi.org/10.1007/BF02620975

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