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Development of a medium for in vitro culture of oocytes from the polychaeteNereis virens

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An Erratum to this article was published on 01 February 1984

Summary

A culture medium was developed that allowed the maintenance and growth of oocytes from the marine polychaeteNereis virens. The ionic composition of the basic salt solution and the medium was similar to that ofNereis coelomic fluid and had a total osmolarity of 920 mOsm. Longevity and the growth of cultured oocytes were investigated with respect to the presence of amino acids andNereis vitellin in the medium. Some oocytes survived for up to 6 wk. In vitro growth occurred at a rate of about 1 μm diam/d (15°C) and cultured cells seemed morphologically and ultrastructurally normal.

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Author notes

  1. A. E. Heacox

    Present address: Department of Zoology, North Dakota State University, 58105, Fargo, North Dakota

Authors and Affiliations

  1. Zoological Institute, University of Köln, Weyertal 119, D-5000, Köln 41, West Germany (FRG)

    A. E. Heacox, A. Fischer & H. -R. Frangenberg

Authors
  1. A. E. Heacox
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  2. A. Fischer
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  3. H. -R. Frangenberg
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Additional information

This work was supported by a grant from the Deutsche Forschungsgemeinschaft, Bonn, West Germany.

An erratum to this article is available at http://dx.doi.org/10.1007/BF02626656.

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Heacox, A.E., Fischer, A. & Frangenberg, H.R. Development of a medium for in vitro culture of oocytes from the polychaeteNereis virens . In Vitro 19, 825–832 (1983). https://doi.org/10.1007/BF02618162

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  • DOI: https://doi.org/10.1007/BF02618162

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