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A continuous-flow method of organ culture

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Summary

A method of perfusion organ culture is described in which explants cultured at the airmedium interface are bathed by a continuous flow of nutrient medium. Morphological studies on the fetal rat lung indicate that explant development in this system is comparable to that obtained using standard organ-culture dishes. Medium supply is easily manipulated and continuous sampling of the effluent stream is possible without disturbing the immediate explant environment. The basic design facilitates secretory-response studies on cultured organ explants as demonstrated by a study of glucose-stimulated insulin release by the neonatal rat pancreas.

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Authors and Affiliations

  1. Department of Anatomy, University of Minnesota, 55455, Minneapolis, Minnesota

    James A. McAteer & Orion D. Hegre

Authors
  1. James A. McAteer
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  2. Orion D. Hegre
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Additional information

This work was supported by U. S. Public Health Service Training Grant No. GM 00114.

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McAteer, J.A., Hegre, O.D. A continuous-flow method of organ culture. In Vitro 14, 795–803 (1978). https://doi.org/10.1007/BF02617974

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