Skip to main content
SpringerLink
Log in

Cellular and viral contributions to maintenance of the SV40-transformed state

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

  1. 1.

    A complex type of intercellular contact, requiring cluster formation among three cells or more, is a prerequisite to growth control for a large number of established fibroblast lines.

  2. 2.

    This form of contact occurs at lowest cell densities among cells that synthesize a specific protein that blocks intercellular WGA agglutination.

  3. 3.

    High saturation density cells, lacking this protein, are rapidly agglutinated by WGA.

  4. 4.

    Low saturation density cells, including a line containing SV40 T antigen and the SV40 genome, make this protein, and consequently are agglutinated poorly.

  5. 5.

    The presence of the SV40 genome and SV40 T antigen are not sufficient to guarantee that a cell lines will have a high saturation density or that it will respond well to the WGA agglutinin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Westphal, H., and R. Dulbecco. 1968. Viral DNA in polyoma and SV40-transformed cell lines. Proc. Natl. Acad. Sci. U. S. A. 59: 1158–1165.

    Article  PubMed  CAS  Google Scholar 

  2. Benjamin, T., 1966. Virus-specific RNA in cells productively infected or transformed by polyoma virus. J. Mol. Biol. 16: 359–373.

    Article  PubMed  CAS  Google Scholar 

  3. Aloni, Y., E. Winocour, and L. Sachs, 1968. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J. Mol. Biol. 31: 415–429.

    Article  PubMed  CAS  Google Scholar 

  4. Dulbecco, R. 1967. The induction of cancer by viruses. Sci. Amer. 216: 28–37.

    Article  PubMed  CAS  Google Scholar 

  5. Todaro, G., and H. Green, 1964. An assay for cellular transformation by SV40. Virology 23: 117–119.

    Article  Google Scholar 

  6. Green, H., and G. Todaro. 1967. In: On the mechanism of transformation of mammalian cells by SV40. Academic Press, New York.

    Google Scholar 

  7. Pollack, R., H. Green, and G. Todaro. 1968. Growth control in cultured cells: selection of sublines with increased sensitivity to contact inhibition and decreased tumor-producing ability. Proc. Natl. Acad. Sci. U. S. A. 60: 126–133.

    Article  PubMed  CAS  Google Scholar 

  8. Rabinowitz, Z., and L. Sachs, 1968. Reversion of properties in cells transformed by polyoma virus. Nature (London) 220: 1203–1206.

    Article  CAS  Google Scholar 

  9. Marin, G., and J. Littlefield. 1968. Selection of morphologically normal cell lines from polyoma transformed BHK hybrids. J. Virol. 2: 69–77.

    PubMed  CAS  Google Scholar 

  10. Rueckert, R., and G. Mueller. 1960. Studies on unbalanced growth in tissue culture. 1. Induction and consequences of thymidine deficiency. Cancer Res. 20: 1584–1591.

    PubMed  CAS  Google Scholar 

  11. Reyes, P., and C. Heidelberger. 1965. Fluorinated pyrimidines XXVI. Mammalian thymidylate synthetase, its mechanism of action and inhibition by fluorinated nucleotides. Mol. Pharm. 1: 14–30.

    CAS  Google Scholar 

  12. Fisher, H., and J. Yeh, 1967. Contact inhibition in colony formation. Science 155: 582–583.

    Article  Google Scholar 

  13. Todaro, G., and H. Green. 1963. Quantitative studies on the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299–313.

    Article  PubMed  CAS  Google Scholar 

  14. Todaro, G., H. Green, and B. Goldberg. 1964. Transformation of properties of an established cell line by SV40 and polyoma virus. Proc. Nat. Acad. Sci. U. S. A. 51: 66–73.

    Article  CAS  Google Scholar 

  15. Stoker, M., M. Shearer, and C. O'Neill. 1968. Growth inhibition of polyoma-transformed cells by contact with static normal fibroblasts. J. Cell Sci. 1: 297–310.

    Google Scholar 

  16. Eagle, H., and F. Levine, 1967. Growth regulatory effects of cellular interactions. Nature (London), 213: 1102–1106.

    Article  CAS  Google Scholar 

  17. Kao, F., and T. Puck. 1968. Genetics of somatic mammalian cells VII. Induction and isolation of nutritional mutants in chinese hamster cells. Proc. Natl. Acad. Sci. U. S. A. 60: 1275–1281.

    Article  PubMed  CAS  Google Scholar 

  18. Luria, S., and M. Delbruck. 1943. Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28: 491–511.

    PubMed  CAS  Google Scholar 

  19. Sanford, K., G. Likely, and W. Earle. 1954. The development of viations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcomaproducing cellsin vitro. J. Natl. Cancer Inst. 15: 215–230.

    PubMed  CAS  Google Scholar 

  20. Defendi, V., J. Lehman, and P. Kraemer. 1963. “Morphologically normal” hamster cells with malignant properties. Virology 19: 592–598.

    Article  PubMed  CAS  Google Scholar 

  21. Abercrombic, M., and E. Ambrose. 1962. The surface properties of cancer cells: a review. Cancer Res. 22: 525–548.

    Google Scholar 

  22. Aaronson, S., and G. Todaro. 1968. Basis for acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024–1026.

    Article  PubMed  CAS  Google Scholar 

  23. Holley, R., and J. Kernan. 1968. “Contact-inhibition” of cell division in 3T3 cells. Proc. Natl. Acad. Sci. U. S. A. 60: 300–304.

    Article  PubMed  CAS  Google Scholar 

  24. Schutz, L., and P. Mora. 1968. The need for direct cell contact in contact inhibition of cell division in culture. J. Cell Physiol. 71: 1–6.

    Article  PubMed  CAS  Google Scholar 

  25. Martz. E. 1969. Ph.D. thesis, Princeton University.

  26. Pardee, A. 1964. Cell division and a hypothesis of cancer. Natl. Cancer Inst. Monogr. 14: 7–20.

    PubMed  CAS  Google Scholar 

  27. Burger, M., and A. Goldberg. 1967. Identification of a tumor-specific determinant on neoplastic cell surfaces. Proc. Nat. Acad. Sci. U. S. A. 57: 359–366.

    Article  CAS  Google Scholar 

  28. Burger, M. 1968. Isolation of a receptor complex for a tumor specific agglutinin from the neoplastic cell surface. Nature (London) 219: 499–500.

    Article  CAS  Google Scholar 

  29. Pollack, R., and M. Burger. 1969. Surfacespecific characteristics of a contact-inhibited cell line containing the SV40 viral genome. Proc. Natl. Acad. Sci. U. S. A. 62: 1074–1076.

    Article  PubMed  CAS  Google Scholar 

  30. Hakomori, I., J. Koscielak, K. Bloch, and R. Jeanluz. 1967. Immunologic relationship between blood group substances and fucose containing glycolipid of human adenocarcinoma. J. Immunol. 98: 31–38.

    PubMed  CAS  Google Scholar 

  31. Burger, M. M. 1968. In: L. Manson (Ed.), Biological properties of the mammalian surface membrance. Wistar Institute Symposium monograph No. 8. Wistar Press, Philadelphia.

    Google Scholar 

  32. Burger, M. 1969. A difference in the architecture of the surface membrane of normal and virally transformed cells. Proc. Natl. Acad. Sci. U. S. A. 62: 994–1001.

    Article  PubMed  CAS  Google Scholar 

  33. Pope, J., and W. Rowe. 1964. Detection of specific antigen in SV40-transformed cells by immunofluorescense. J. Exp. Med. 120: 121–128.

    Article  PubMed  CAS  Google Scholar 

  34. Gilden, R., R. Carp, F. Tajuchi, and V. Defendi. 1965. The nature and localization of the SV40 induced complement-fixing antigen. Proc. Natl. Acad. Sci. U. S. A. 53: 684–692.

    Article  PubMed  CAS  Google Scholar 

  35. Todaro, G., and K. Takemoto. 1969. “Rescued” SV40: increased transforming efficiency in mouse and human cells. In press.

  36. Pollack. R. Unpublished experiments.

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, New York University Medical Center, New York, New York

    Robert E. Pollack

Authors
  1. Robert E. Pollack
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pollack, R.E. Cellular and viral contributions to maintenance of the SV40-transformed state. In Vitro 6, 58–65 (1970). https://doi.org/10.1007/BF02616134

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02616134

Keywords

Search

Navigation