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Serologic and karyologic evidence of incorrect identity of an animal cell line (guinea pig spleen)

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Summary

A cell line labeled GPS and presumed to be guinea pig spleen has been shown to be mouse and to carry the H-2k allele. Identification of characteristic chromosome markers suggests that the proper identity may be L-M mouse cells. The use of immunologic and karyologic identification procedures for routine checking of cell lines is discussed.

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Author notes

  1. Philip R. Herrick (Medical Student Research Fellow American Cancer Society IN-40)

    Present address: , 208 New London Road, Chelmsford, Essex, UK

  2. Donald J. Merchant

    Present address: W. Alton Jones Cell Science Center, P. O. Box 631, 12946, Lake Placid, New York

  3. Marcia C. Shearer

    Present address: Smith, Kline, and French Laboratories, 1500 Spring Garden Street, 19191, Philadelphia, Pennsylvania

Authors and Affiliations

  1. Department of Microbiology, Medical School, The University of Michigan, 48104, Ann Arbor, Michigan

    Philip R. Herrick (Medical Student Research Fellow American Cancer Society IN-40), Gregory W. Baumann, Donald J. Merchant, Marcia C. Shearer, Chrrles Shipman Jr. & Robert G. Brackett

  2. Department of Oral Biology, School of Dentistry, The University of Michigan, 48104, Ann Arbor, Michigan

    Philip R. Herrick (Medical Student Research Fellow American Cancer Society IN-40), Gregory W. Baumann, Donald J. Merchant, Marcia C. Shearer, Chrrles Shipman Jr. & Robert G. Brackett

  3. Research Laboratories, Parke, Davis and Company, 48232, Detroit, Michigan

    Philip R. Herrick (Medical Student Research Fellow American Cancer Society IN-40), Gregory W. Baumann, Donald J. Merchant, Marcia C. Shearer, Chrrles Shipman Jr. & Robert G. Brackett

Authors
  1. Philip R. Herrick
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  2. Gregory W. Baumann
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  3. Donald J. Merchant
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  4. Marcia C. Shearer
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  5. Chrrles Shipman Jr.
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  6. Robert G. Brackett
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Additional information

This work was supported in part by United States Public Health Service Grant CA03720.

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Herrick, P.R., Baumann, G.W., Merchant, D.J. et al. Serologic and karyologic evidence of incorrect identity of an animal cell line (guinea pig spleen). In Vitro 6, 143–147 (1970). https://doi.org/10.1007/BF02616115

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  • DOI: https://doi.org/10.1007/BF02616115

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