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Clonal isolation of differentiated rat lung cells

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A number of diploid clones have been isolated from an enzymatic dispersion of normal rat lung. Four of these clones are epithelial in morphology, the remainder fibroblastic. On the basis of electron microscopic observations, two of the epithelial clones appear to have originated from type II alveolar pneumonocytes.

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References

  1. Kikkawa, Y., E. K. Motoyama, and C. D. Cook. 1965. The ultrastructure of the lungs of lambs. Am. J. Pathol. 47: 877–903.

    PubMed  CAS  Google Scholar 

  2. Askin, F. B., and C. Kuhn. 1971. Cellular origin of pulmonary surfactant. Lab. Invest. 25: 260–268.

    PubMed  CAS  Google Scholar 

  3. Redding, R. A., W. H. J. Douglas, and M. Stein. 1972. Thyroid hormone influence upon lung surfactant metabolism. Science 175: 994–996.

    Article  PubMed  CAS  Google Scholar 

  4. Hayflick, L., and P. S. Moorhead. 1961. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585–621.

    Article  Google Scholar 

  5. Konigsberg, I. R.. 1968. Clonal analysis of myogenesis. Science 140: 1273–1284.

    Article  Google Scholar 

  6. Coon, H. G. 1966. Clonal stability and phenotypic expression of chick cartilage cells in vitro. Proc. Natl. Acad. Sci. U.S.A. 55: 66–73.

    Article  PubMed  CAS  Google Scholar 

  7. Cahn, R. D., and M. B. Cahn. 1966. Heritability of cellular differentiation: clonal growth and expression of differentiation in retinal pigment cells in vitro. Proc. Natl. Acad. Sci. U.S.A. 55: 106–114.

    Article  PubMed  CAS  Google Scholar 

  8. Spooner, B. S. 1970. The expression of differentiation by chick embryo thyroid in cell culture I. Functional and fine structural stability in mass and clonal culture. J. Cell. Physiol. 75: 33–47.

    Article  PubMed  CAS  Google Scholar 

  9. Kaighn, M. E., and A. M. Prince. 1971. Production of albumin and other serum proteins by clonal cultures of normal human liver. Proc. Natl. Acad. Sci. U.S.A. 68: 2396–2400.

    Article  PubMed  CAS  Google Scholar 

  10. Tashjian, A. H., Y. Yasumura, L. Levine, G. H. Sato, and M. L. Parker. 1968. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342–352.

    Article  PubMed  CAS  Google Scholar 

  11. Tashjian, A. H., F. C. Bancroft, and L. Levine. 1970. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells: differential effects of hydrocortisone and tissue extracts. J. Cell Biol. 47: 61–70.

    Article  PubMed  CAS  Google Scholar 

  12. Kaighn, M. E. 1973. Human liver cells. In: P. F. Kruse, Jr. and M. K. Patterson (Eds.),Tissue Culture: Methods and Applications. Academic Press, New York, pp. 54–58.

    Google Scholar 

  13. Kaighn, M. E. Birth of a culture—source of postpartum anomalies. Presented at a National Cancer Institute Symposium:New Horizons for Tissue Culture in Cancer Research, Dec. 3–4, 1973. J. Natl. Cancer Inst., in press.

  14. Steinberg, M. S. 1963. “ECM”: its nature, origin and function in cell aggregation. Exp. Cell Res. 30: 257–279.

    Article  PubMed  CAS  Google Scholar 

  15. Douglas, W. H. J., and J. Elser. 1972. A method for in situ embedding of cultured cells grown on plastic surfaces. In Vitro 8: 26–29.

    PubMed  CAS  Google Scholar 

  16. Page-Roberts, B. A.. 1972. Preparation and partial characterization of a lamellar body fraction from rat lung. Biochim. Biophys. Acta 260: 334–338.

    PubMed  CAS  Google Scholar 

  17. Kruse, P. F., Jr., L. N. Keen, and W. L. Whittle. 1970. Some distinctive characteristics of high density perfusion cultures of diverse cell types. In Vitro 6: 75–88.

    PubMed  Google Scholar 

  18. Evans, M. J., L. J. Cabral, R. J. Stevens, and G. Freeman. 1973. Renewal of alveolar epithelium in the rat following exposure to NO2. Am. J. Pathol. 70: 175–198.

    PubMed  CAS  Google Scholar 

  19. Macpherson, I. 1973. Soft agar techniques. In: P. F. Kruse, Jr. and M. K. Patterson (Eds.),Tissue Culture: Methods and Applications. Academic Press, New York, pp. 276–280.

    Google Scholar 

  20. Montagnier, L. 1968. Correlation entre la transformation des cellules BHK 21 et leur résistance aux polysaccharides acides en milieu gelifié. C. R. Acad. Sci. (D) (Paris) 267: 921–924.

    CAS  Google Scholar 

  21. Hayflick, L. 1965. Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med. 23: 285–303.

    PubMed  Google Scholar 

  22. Douglas, W. H. J. 1973. A whole cell mount technique for detecting mycoplasma on the surface of cultured cells. In Vitro 8: 413.

    Google Scholar 

  23. Ham, R. G. 1965. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. U.S.A. 53: 288–293.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. W. Alton Jones Cell Science Center, P.O. Box 631, 12946, Lake Placid, New York

    William H. J. Douglas & M. Edward Kaighn

Authors
  1. William H. J. Douglas
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  2. M. Edward Kaighn
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Supported by funds from the W. Alton Jones Foundation and the American Lung Association.

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Douglas, W.H.J., Kaighn, M.E. Clonal isolation of differentiated rat lung cells. In Vitro 10, 230–237 (1974). https://doi.org/10.1007/BF02615237

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  • DOI: https://doi.org/10.1007/BF02615237

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