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The estimation of free light chains of immunoglobulins in biological fluids

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International Journal of Clinical and Laboratory Research

Summary

Methods for the estimation of the free light chains of immunoglobulins in serum, urine and cerebrospinal fluid are divided into two groups, electrophoretic and immunological, and the analytical performence of each method described. The problems associated with the accurate and precise determination of free light chains by the different methods are discussed and their complementary clinical roles emphasized. It is proposed that an International Reference Preparation for free light chains is established.

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References

  1. Artiss JD, Epstein E, Kiechle FL, Zak B Potential problems in serum protein electrophoresis. Clin Lab Med 6:427, 1986

    PubMed  CAS  Google Scholar 

  2. Austin GE, Check IJ, Hunter RL. Analysis of the reliability of serial paraprotein determinations in patients with plasma cell dyscrasias. Am J Clin Pathol 79:227, 1982

    Google Scholar 

  3. Axiak SM, Krishnamoorthy L, Guinan J, Raison RL, Quantitation of free ϰ light chains in serum and urine using a monoclonal antibody based inhibition enzyme-linked immunoassay. J Immunol Methods 99:141, 1987

    Article  PubMed  CAS  Google Scholar 

  4. Berggård I, Edelman GM, Normal counterparts to Bence-Jones proteins: free L polypeptide chains of human γ-globulin. Proc Natl Acad Sci USA 49:330, 1963

    Article  PubMed  Google Scholar 

  5. Berggård I, Peterson PA, Immunoglobulin components in normal human urine. Nobel Symp. 3:71, 1967

    Google Scholar 

  6. Berggård I, Peterson PA, Polymeric forms of free normal kappa and lambda chains of human immunoglobulins. J Biol Chem 244:4299, 1969

    PubMed  Google Scholar 

  7. Brouwer J, Otting-van der Ruit MO, Busking-van der Lely HB, Estimation of free light chains of immunoglobulins by enzyme immunoassay. Clin Chim Acta 150:267, 1985

    Article  PubMed  CAS  Google Scholar 

  8. Cole PW, Durie BGM, Salmon SE, Immunoquantitation of free light chain immunoglobulins: application in multiple myeloma. J Immunol Methods 19:341, 1978

    Article  PubMed  CAS  Google Scholar 

  9. Dammaco F, Waldenström J, Serum and urine light chain levels in benign monoclonal gammopathies, multiple myeloma and Waldenström's macroglobulinaemia. Clin Exp Immunol 3:911, 1968

    Google Scholar 

  10. Epstein WV, Tan M, Increase of L-chain proteins in the sera of patients with systemic lupus erythematosus and the synovial fluids of patients with peripheral rheumatoid arthritis. Arthritis Rheum 9:713, 1966

    Article  PubMed  CAS  Google Scholar 

  11. Fagnart OC, Sindic CJM, Laterre C, Free kappa and lambda light chains in the cerebrospinal fluid of patients with multiple sclerosis and other neurological diseases. J Neuroimmunol 19:119, 1988

    Article  PubMed  CAS  Google Scholar 

  12. Gallo P, Tavalato B, Bergenbrant S, Siden A, Immunoglobulin light chains in the cerebrospinal fluid. A study with special reference to the occurrence of free light chains in cerebrospinal fluid with and without oligoclonal immunoglobulin G. J Neurol Sci 94:241, 1989

    Article  PubMed  CAS  Google Scholar 

  13. Heino J, Rajamäki A, Irjala K, Turbidimetric measurement of Bence-Jones proteins using antibodies against free light chains of immunoglobulins. An artifact caused by different polymeric forms of light chains. Scand J Clin Lab Invest 44:173, 1984

    Article  PubMed  CAS  Google Scholar 

  14. Hemmingsen L, Skaarup P, The 24th excretion of plasma proteins in the urine of apparently healthy subjects. Scand J Clin Lab Invest 35:347, 1975

    Article  PubMed  CAS  Google Scholar 

  15. Hobbs JR, Bence-Jones proteins. In: Marks V, Hales CN (eds) Essays in Medical Biochemistry. The Biochemical Society and The Association of Clinical Biochemists, London 1:105–131, 1975

    Google Scholar 

  16. Jefferis R, Deverill I, Ling NR, Reeves WG, Quantitation of human total IgG, kappa IgG and lambda IgG in serum using monoclonal antibodies. J Immunol Method. 39:355, 1980

    Article  CAS  Google Scholar 

  17. Keren DF, Di Sante AC, Bordine SL, Densitometric scanning of high-resolution electrophoresis of serum: methodology and clinical application. Am J Clin Pathol 85:348, 1986

    PubMed  CAS  Google Scholar 

  18. Kohn J, Cellulose acetate electrophoresis and immunoelectrophoresis. In: Smith I (ed) Chromatographic and electrophoretic techniques, vol 2. Heinemann, London, pp 90–137, 1976

    Google Scholar 

  19. Laurell CB, Complexes formed in vivo between immunoglobulin light chain kappa, prealbumin and/or alpha1-antitrypsin. Immunochemistry 7:461, 1970

    Article  PubMed  CAS  Google Scholar 

  20. Ling NR, Lowe J, Hardie D, Evans S, Jefferis R, Detection of free ϰ chains in human serum and urine using pairs of monoclonal antibodies reacting with Cϰ epitopes not available on whole immunoglobulins. Clin Exp Immunol 52:234, 1983

    PubMed  CAS  Google Scholar 

  21. Lolli F, Amaducci L, Measurement of free kappa immunoglobulin light chains in the cerebrospinal fluid by a competitive avidin-biotin ELISA. Clin Chim Acta 182:229, 1989

    Article  PubMed  CAS  Google Scholar 

  22. Mehta PD, Cook SD, Troiano MD, Coyle MD, Increased free light chains in the urine from patients with multiple sclerosis. Neurology 41:540, 1991

    PubMed  CAS  Google Scholar 

  23. Peterson PA, Berggård I, Urinary immunoglobulin components in normal, tubular and glomerular proteinuria: quantities and characteristics of free light chains, IgG, IgA, and Fc fragment. Eur J Clin Invest 1:255, 1971

    Article  PubMed  CAS  Google Scholar 

  24. Riberi M, Bernard D, Depieds R, Evidence for the presence of lambda chain dimers in cerebrospinal fluid of patients suffering from subacute sclerosing panencephalitis. Clin Exp Immunol 19:45, 1975

    PubMed  CAS  Google Scholar 

  25. Riches PG, Michael D, Sheldon J, Weatherald L, Sensitive colloidal metal staining of cerebrospinal fluid proteins in agarose gels, Lab Pract 35:112, 1986

    CAS  Google Scholar 

  26. Righetti PG, Casero P, Del Campo GB, Gold staining on cellulose acetate membranes. Clin Chim Acta 157:1676, 1986

    Article  Google Scholar 

  27. Robinson EL, Gowland E, Ward ID, Scarffe JH, Radio-immunoassay of free light chains of immunoglobulins in urine. Clin Chem 28:2254, 1982

    PubMed  CAS  Google Scholar 

  28. Rohringer R, Holden D, Protein blotting: detection of proteins with colloidal gold, and of glycoproteins and lectins with biotin-conjugated and enzyme probes. Anal Biochem 144:118, 1984

    Article  Google Scholar 

  29. Rudick RA, Peter DR, Bidlack JM, Knutson DW, Multiple sclerosis: free light chains in cerebrospinal fluid. Neurology 35:1443, 1985

    PubMed  CAS  Google Scholar 

  30. Rudick RA, Pallant A, Bidlack JM, Herndon RM, Free kappa light chains in multiple sclerosis spinal fluid. Ann Neurol 20:63, 1986

    Article  PubMed  CAS  Google Scholar 

  31. Sindic CJM, Delacroix DL, Vaerman JP, Laterre EC, Masson PL. Study of IgA in the cerebrospinal fluid of neurological patients with special reference to size, subclass and local production. J Neuroimmunol 7:65, 1984

    Article  PubMed  CAS  Google Scholar 

  32. Smith AM, Thompson RA. Paraprotein estimation: a comparison of immunochemical and densitometric techniques. J Clin Pathol 31:1156, 1978

    Article  PubMed  CAS  Google Scholar 

  33. Sølling K, Free light chains in normal serum and urine determined by radioimmunoassay. Scand J Clin Lab Invest 35:407, 1975

    Article  PubMed  Google Scholar 

  34. Sølling K, Polymeric forms of free light chains in serum from normal individuals and from patients with renal diseases. Scand J Clin Lab Invest 36:447, 1976

    Article  PubMed  Google Scholar 

  35. Sølling K, Normal values for free light chains in serum in different age groups. Scand J Clin Lab Invest 37:21, 1977

    Article  PubMed  Google Scholar 

  36. Sølling K, Light chain polymerism in normal individuals, in patients with severe proteinuria and in patients with inhibited tubular protein reabsorption by lysine. Scand J Clin Lab Invest 40:129, 1980

    Article  PubMed  Google Scholar 

  37. Sølling K, Sølling J, Nielsen JL, Polymeric Bence Jones proteins in serum in myeloma patients with renal insufficiency. Acta Med Scand 216:495, 1984

    Article  PubMed  Google Scholar 

  38. Solomon A, Bence-Jones proteins and light chains of immunoglobulins. N Engl J Med 294:17, 1976

    Article  PubMed  CAS  Google Scholar 

  39. Solomon A, Bence-Jones proteins and light chains of immunoglobulins. N Engl J Med 294:91, 1976

    Article  PubMed  CAS  Google Scholar 

  40. Solomon A, Review: light chains of immunoglobulins: Structural-genetic correlates. Blood 68:603, 1986

    PubMed  CAS  Google Scholar 

  41. Stemerman D, Papadea C, Martino-Saltzman D, O'Connell AC, Demaline B, Austin GEA, Precision and reliability of paraprotein determinations by high-resolution agarose gel electrophoresis. Am J Clin Pathol 81:435, 1989

    Google Scholar 

  42. Tan M, Epstein WV, A direct immunologic assay of human sera for Bence-Jones proteins (L-chains). J Lab Clin Med 66:344, 1965

    PubMed  CAS  Google Scholar 

  43. Teppo AM, Groop L, Urinary excretion of light chains in diabetic subjects. Increased excretion of kappa light chains in diabetic patients with and without proliferative retinopathy. Diabetes 34:589, 1985

    Article  PubMed  CAS  Google Scholar 

  44. Tillyer CR, Iqbal J, Raymond J, Gore M, McIlwain TJ, Immunoturbidimetric assay for estimating free light chains of immunoglobulins in urine and serum. J Clin Pathol 44:466, 1991

    Article  PubMed  CAS  Google Scholar 

  45. Vaughan J, Jacox RF, Gray BA, Light and heavy chain components of gamma globulins in urines of normal persons and patients with agammaglobulinaemia. J Clin Invest 46:266, 1967

    PubMed  CAS  Google Scholar 

  46. Whicher JT, Warren C, Chambers RE, Immunochemical assays for immunoglobulins. Ann Clin Biochem 21:78, 1984

    PubMed  CAS  Google Scholar 

  47. Whicher JT, Wallage M, Fifield R, Use of immunoglobulin heavy and light chain measurements compared with existing techniques as a means of typing monoclonal immunoglobulins. Clin Chem 33:1771, 1987

    PubMed  CAS  Google Scholar 

  48. Zelkowitz L, Yakulis V, Immunologic methods for detection and quantification of Bence-Jones proteins. J Lab Clin Med 76:973, 1970

    PubMed  CAS  Google Scholar 

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  1. Department of Chemical Pathology, Royal Marsden Hospital, Fulham Road, SW3 6JJ, London, UK

    C. R. Tillyer

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Tillyer, C.R. The estimation of free light chains of immunoglobulins in biological fluids. Int J Clin Lab Res 22, 152–158 (1992). https://doi.org/10.1007/BF02591415

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