Summary
The uptake of 3-O-[14C]methyl-D-glucose was measured in erythrocytes of normal and non-insulin-dependent diabetic subjects. In normal subjects, the uptake of the sugar was rapid, saturable, temperature-sensitive and inhibited by cytochalasin B. Over 30 s incubation at 20°C, the uptake of 3-O-[14C]methyl-D-glucose (20 mM) was lower in diabetic than in normal subjects. These findings raise the view that an alteration of hexose transport into insulin-insensitive cells may participate to the perturbation of glucose homeostasis in diabetic patients.
Similar content being viewed by others
References
Jacobs D. B., Hayes G. R., Lockwood D. H.: In vitro effects of sulfonylureas on glucose transport and translocation of glucose transporters in adipocytes from streptozotocin-induced diabetic rats — Diabetes38, 205, 1989.
Malaisse-Lagae F., Giroix M.-H., Sener A., Malaisse W. J.: Phosphorylation of 3-O-methyl-D-glucose by yeast and beef hexokinase — FEBS Letters198, 292, 1986.
Marynissen G., Sener A., Malaisse W. J.: D-glucose uptake by pancreatic islets from normal and hyperglycemic rats — Med. Sci. Res.16, 1181, 1988.
Mueckler M.: Family of glucose-transporter genes: implications for glucose homeostasis and diabetes — Diabetes39, 6, 1990.
Naftalin R. J., Smith P. M., Roselaer S. E.: Evidence for non-uniform distribution of D-glucose within human red cells during net exit and counterflow — Biochim. biophys. Acta (Amst.)820, 235, 1985.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gomis, R., Tomas, C., Novials, A. et al. 3-O-methyl-D-glucose uptake by erythrocytes of normal and diabetic subjects. Acta diabet. lat 27, 279–283 (1990). https://doi.org/10.1007/BF02580931
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02580931