Abstract
Chondrocytes were isolated from growth cartilage and resting cartilage of rat rib and cultivatedin vitro. The cultivated chondrocytes were placed in Millipore diffusion chambers, which were then implanted into the abdominal cavities of rats for several weeks and prepared for histological analysis. The results indicate that growth cartilage cells have a remarkable osteogenic potential, even after cultivationin vitro, whereas resting cartilage cells show no osteogenic activity. However, growth cartilage cells alone do not form new bone but require the participation of certain host cells to initiate osteogenic differentiation.
References
Bentley, G., Greer, R. B.: The fate of chondrocytes in endochondral ossification in the rabbit. J. Bone Jt. Surg. B52, 571–577 (1970).
Bloom, W., Fawcett, D. W., A textbook of histology, 9th ed., p. 212–262, Philadelphia-London-Toronto: W. B. Saunders Co. 1968
Brighton, C. T., Sugioka, Y., Hunt, R.: Cytoplasmic structures of epiphyseal plate chondrocytes; Quantitative evaluation using electron micrographs of rat costochondral junctions with special reference to the fate of hypertrophic cells. J. Bone Jt. Surg. A55, 771–784 (1973).
Cahn, R. D., Coon, H. G., Cahn, M. B.: Growth of differentiated cells: Cell culture and cloning techniques. In: Methods in developmental biology, (Wilt, F., Wessells, N. K., eds.), p. 493–530, New York: Thomas Crowell 1967
Coon, H. G.: Clonal stability and phenotypic expression of chick cartilage cells. Proc. nat. Acad. Sci. (Wash.)55, 66–73 (1966).
Gillette, R., Mardfin, D., Schour, I.: Osteogenesis in subcutaneous rib transplants between normal andia rats. Amer. J. Anat.99, 447–471 (1956)
Green, W. T., Jr.: Behavior of articular chondrocytes in cell culture. Clin. Orthop.75, 248–260 (1971)
Ham, A. W.: Histology, 5th ed., p. 373–450. Philadelphia and Montreal: J. B. Lippincott Co. 1965
Ham, R. G., Sattler, G. L.: Clonal growth of differentiated rabbit cartilage cells. J. cell. Physiol.72, 109–114 (1968)
Holtrop, M. E.: The origin of bone cells in endochondral ossification. In: Calcified tissues, (Fleisch, H., ed.), p. 32–36. Berlin-Heidelberg-New York: Springer 1966
Holtrop, M. E.: The potencies of the epiphyseal cartilage in endochondral ossification. Proc. kon. ned. Akad. Wet. Ser. C70, 29–28 (1967)
Holtrop, M. E.: Factors influencing the growth rate in endochondral ossification. Proc. kon. ned. Akad. Wet. Ser. C70, 29–38 (1967)
Holtrop, M. E.: The ultrastructure of the epiphyseal plate. I. The flattened chondrocytes. Calcif. Tiss. Res.9, 131–139 (1972)
Holtrop, M. E.: The ultratructure of the epiphyseal plate. II. The hypertrophic chondrocytes. Calcif. Tiss. Res.9, 140–151 (1972)
Holtzer, H., Abbott, J., Lash, J., Holtzer, S.: The loss of phenotypic traits by differentiated cellsin vitro. I. Dedifferentiation of cartilage cells. Proc. nat. Acad. Sci. (Wash.)46, 1533–1542 (1960)
Kuhlman, R. E., McNamee, M. J.: The biochemical importance of the hypertrophic cartilage cell area to enchondral bone formation. J. Bone Jt. Surg. A52, 1025–1033 (1970)
Lacroix, P.: The organization of bones, p. 1–235. London: J. A. Churchill 1951
Levenson, G. E.: The effect of ascorbic acid on monolayer cultures of three types of chondrocytes. Exp. Cell Res.55, 225–228 (1969)
Shimomura, Y., Ray, R. D.: The fate of the hypertrophic cells in the growth cartilage I. Transplantation of the growth cartilage. Cent. Jap. J. orthop. traumat. Surg.16, 726–728 (1973).
Silberman, M., Frommer, J.: Ultrastructure of developing cartilage in the mandibular condyle of the mouse. Acta anat. (Basel)90, 330–346 (1974)
Sissons, H. A.: The growth of bone. In: Biochemistry and physiology of bone (Bourne, G. H., ed.), vol. III, p. 145–180, New York: Academic Press, 1971
Trueta, J.: The role of the vessels in osteogenesis. J. Bone Jt. Surg. B45, 402–418 (1963)
Urist, M. R., McLean, F. C.: Osteogenic potency and new bone formation by induction in transplants to the anterior chamber of the eye. J. Bone Jt. Surg. A34, 443–470 (1952)
Urist, M. R.: Bone formation by autoinduction. Science150, 893–899 (1965)
Urist, M. R., Dowell, T. A., Hay, P. H., Strates, B. S.: Inductive substrates for bone formation. Clin. Orthop.59, 59–96 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shimomura, Y., Yoneda, T. & Suzuki, F. Osteogenesis by chondrocytes from growth cartilage of rat rib. Calc. Tis Res. 19, 179–187 (1975). https://doi.org/10.1007/BF02564002
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02564002