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Cultivation of human keratinocyte stem cells: current and future clinical applications

  • Cellular Engineering: Bioengineering of the Skin
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Abstract

Cultured human keratinocytes have a wide spectrum of clinical applications. Clinical results reported by several investigators are, however, contradictory. In this review, the authors discuss the biological and surgical issues which play a key role in the clinical outcome of cultured epidermal autografts used for the treatment of massive full-thicknes burns. The importance of cultivation of epidermal stem cells and of their transplantation onto a wound bed prepared with donor dermis is emphasised. The paper also reviews recent data showing that: (i) cultured epidermal autografts bearing melanocytes can be used for the treatment of stable vitiligo; (ii) keratinocytes isolated from other lining epithelia, such as oral, urethral and corneal epithelia, can be cultivated and grafted onto patients suffering from disabling epithelial defects; (iii) keratinocyte stem cells can be stably transduced with retroviral vectors and are therefore attractive targets for the gene therapy of genodermatoses.

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  1. Laboratory of Tissue Engineering, I.D.C.-IRCCS, Istituto Dermopatico dell'Immacolata, Via dei Castelli Romani, 83/85, 00040, Pomezia, Roma, Italy

    G. Pellegrini, S. Bondanza, L. Guerra &  M. De Luca

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Pellegrini, G., Bondanza, S., Guerra, L. et al. Cultivation of human keratinocyte stem cells: current and future clinical applications. Med. Biol. Eng. Comput. 36, 778–790 (1998). https://doi.org/10.1007/BF02518885

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