Abstract
The metaphase I and anaphase I stages of meiosis of wheatxrye hybrids carrying theph1b mutation were analyzed by genomic in situ hybridization. This technique allows distinction between three different types of wheat-rye associations in metaphase I configurations as well as detection of wheat-rye recombinant chromosomes in anaphase I cells. The frequency of associations between wheat and rye chromosomes greatly exceeded the level of wheat-rye recombination found in the three hybrids examined. Extremely distal associations, which account for about 50% of the total wheat-rye metaphase I chromosomal pairing, can explain such a discrepancy between metaphase I and anaphase I data. It is further discussed whether these associations reflect very distally located chiasmata or nonchiasmatic pairing. The sizes of the segments exchanged in wheat-rye recombinant chromosomes provide cytological evidence that wheat-rye recombination is restricted to the distal chromosomal regions.
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Edited by: P.B. Moens
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Benavente, E., Fernández-Calvín, B. & Orellana, J. Relationship between the levels of wheat-rye metaphase I chromosomal pairing and recombination revealed by GISH. Chromosoma 105, 92–96 (1996). https://doi.org/10.1007/BF02509518
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DOI: https://doi.org/10.1007/BF02509518