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Determination of organochlorine compounds in human adipose tissue using automated liquid chromatographic clean up and gas chromatography—tandem mass spectrometry

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Summary

A multiresidue method based on automated normal-phase LC clean-up and GC-MS-MS analysis has been developed for the determination of organochlorine pesticides and polychlorinated biphenyls in human adipose tissues. Analytes were extracted by dissolving the fat samples in n-hexane, and the hexane extracts were injected onto a silica gel column for automated LC clean-up prior to analysis by GC-MS-MS. The high efficiency of the HPLC clean up for the elimination of fats in combination and the use of GC with tandem mass spectrometry detection gave detection limits at the low ng g−1 concentration level for all analytes using as little as 0.25 g of sample.

The overall analytical procedure, was applied to several human abdominal fat tissue samples: HCB, β-HCH, p,p′-DDE and the higher chlorinated PCBs (138, 153 and 180) were the most frequently detected compounds, at levels of 26–1273 ng g−1, 19–933 ng g−1, 121–3945 ng g−1 and 56–496 ng g−1, respectively.

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Authors and Affiliations

  1. Analytical Chemistry, Dept. Experimental Sciences, University Jaume I, 12004, Castellón, Spain

    F. Hernández, E. Pitarch, R. Serrano & C. Guerrero

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  1. F. Hernández
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  2. E. Pitarch
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  3. R. Serrano
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  4. C. Guerrero
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Hernández, F., Pitarch, E., Serrano, R. et al. Determination of organochlorine compounds in human adipose tissue using automated liquid chromatographic clean up and gas chromatography—tandem mass spectrometry. Chromatographia 55, 715–722 (2002). https://doi.org/10.1007/BF02491787

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  • DOI: https://doi.org/10.1007/BF02491787

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