Abstract
An experiment was made to identify the lymphoid cells by the use of indirect immunofluorescent staining with rabbit antiserum prepared against P3-J lymphoblastoid cells of Burkitt origin. Cultured lymphoblastoid cell line P3-J, Raji, RPMI #8226 and RPMI #4666 were stained positively with this antiserum. Nonlymphoid cell line RPMI #41 was negative for the staining. Lymphatic cells of stamped preparations from human lymph nodes were also positive for the staining. When human peripheral white cell smears were tested, only lymphocytic cells were positively stained.
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Coons, A. H.: General cytochemical method 1, Academic Press, N. Y., 399–422, 1958.
Epstein, M. A., Achong, B. G., Barr, Y. M., Zajac, B., Henle, G. and Henle, W.: Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji), J. Nat. Cancer Inst. 37: 547–559, 1966.
Kamei, H. and Moore, G. E.: Production of phage-neutralizing activity in vitro by cells derived from Burkitt's lymphoma, Nature 215: 860–861, 1967.
Kamei, H. and Moore, G. E.: Antibody production stimulated in vitro in Burkitt lymphoma cells, J. Immunol. 101: 587–593, 1968.
Kamei, H. and Moore, G. E.: Antibody production in cultured lymphoblastoid cell line, Experientia 24: 410–411, 1969.
Matsuoka, Y., Moore, G. E., Yagi, Y. and Pressman, D.: Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma, Proc. Soc. 125: 1246–1250, 1968.
Monaco, A. P., Wood, M. L. and Russel, P. S.: Some effects of purified heterologous anti-human lymphocyte serum in man, Transplantation 5: 1106–1114, 1967.
Koike, A., Moore, G. E., Mendosa, C. B. and Watne, A. L.: Heterologous, homologous and autologous transplantation of human tumors, Cancer 16: 1065–1071, 1963.
Najarian, J. S., Simmons, R. L., Gewurz, H., Moberg, A., Merkel, F. and Moore, G. E.: Anti-serum to cultured human lymphoblasts; preparation, purification and immunosuppressive properties in man, Ann. Surg. 170: 617–632, 1969.
Pulvertaft, R. J. V.: A study of malignant tumors in Nigeria by short-term tissue culture, J. Clin. Pathol. 18: 261–273, 1965.
Takahashi, M., Tanigaki, N., Yagi, Y., Moore, G. E. and Pressman, D.: Presence of two different immunoglobulins heavy chains in individual cells of established human hematopoietic cell lines, J. Immunol. 100: 1176–1183, 1968.
Tanigaki, N., Yagi, Y., Moore, G. E. and Pressman, D.: Immunoglobulin production in human leukemia cell lines, J. Immunol. 97: 634–646, 1966.
Toshima, S., Takagi, N., Minowada, J., Moore, G. E. and Sandberg, A. A.: Electron microscopic and cytogenetic studies of cells derived from Burkitt's lymphoma, Cancer Research 27: 753–771, 1967.
Waksman, B. H., Arbouys, S. and Arnason, B. G.: The use of specific “lymphocyte” antisera to inhibit hypersensitive reactions of the “delayed” type, J. Exp. Med. 114: 997–1022, 1961.
Woodruff, M. F. A., Reid, B. and James, K.: Effect of antilymphocytic antibody and antibody fragments on human lymphocytes in vitro, Nature 215: 591–594, 1967.
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Kamei, H., Hoshikawa, S. Identification of lymphoid cells by the use of immunofluorescent staining with antiserum against cultured lymphoblastoid cells. The Japanese Journal of Surgery 2, 73–76 (1972). https://doi.org/10.1007/BF02468934
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DOI: https://doi.org/10.1007/BF02468934