Summary
A chromatographic method is described for the isolation of the methionine-bound sulfur in casein for stable-isotope-ratio analysis. Casein is cleaved by acid hydrolysis and the high-molecular-weight secondary products formed are removed by adsorption on octadecyl-silica. Methionine is separated from the bulk of the polar amino acids by cation-exchange chromatography and subsequently oxidized to methionine sulfone by performic and peracetic acid. In a further step the methionine is isolated from the accompanying nonpolar amino acids by cation-exchange chromatography with a mobile phase of very low eluent strength.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. Mosandl, Food Rev. Int.11, 595 (1995).
G. Lamprecht, F. Pichlmayer, E. R. Schmid, J. Agric. Food Chem.42, 1722 (1994).
B. E. Kornexl, A. Roßmann, H.-L. Schmidt, Z. Lebensm. Unters. Forsch.202, 55 (1996).
G. G. Martin, V. Hanote, M. Lees, Y. L. Martin, J. Assoc. Off. Anal. Chem.79, 62 (1996).
A. Roßmann, C. Lüllmann, H.-L. Schmidt, Z. Lebensm. Unters, Forsch.195, 307 (1992)
J. W. White, J. Assoc. Off. Anal. Chem.75, 543 (1992).
M. P. Day, B. Zhang, G. J. Martin, J. Sci. Food Agric.67, 113 (1995).
E. R. Schmid, I. Fogy, P. Schwarz, Z. Lebensm. Unters. Forsch.166, 89 (1978).
E. R. Schmid, H. Grundmann, I. Fogy, W. Papesch, D. Rank, Biomed. Mass Spectrom.8, 496 (1981).
F. Pichlmayer, K. Blochberger, Austrian Research Center Publ. OEFZS 4610, (1991).
W. N. Eigel, J. E. Butler, C. A. Ernstrom, M. H. Farrell, V. R. Harwalkar, R. Jenness, R. M. Whiney, J. Dairy Sci.67, 1599 (1984).
R. L. Cunico, T. Schlabach, J. Chromatogr.266, 461 (1983).
G. J. Hughes, K. J. Wilson, J. Chromatogr.242, 337 (1982).
S. Ganno, Y. Hamano, J. Kobayashi, T. Masaki, J. Chromatogr.332, 275 (1985).
S. E. Moller, J. Chromatogr.613, 223 (1993).
L. Lemieux, J. Amiot, J. Liq. Chromatogr.13, 4023 (1990).
M. Roth, Anal. Chem.43, 880 (1971).
K. Blau, J. Halkel, Handbook of Derivatives for Chromatography, Wiley & Sons, Chichester, 1993.
H. Frister, H. Meisel, E. Schlimme, Fresenius' Z. Anal. Chem.330, 631 (1988).
F. Pichlmayer, K. Blochberger, Fresenius' Z. Anal. Chem.331, 196 (1988).
G. Lamprecht, J. Chromatogr. B691, 297 (1997).
S. Moore, J. Biol. Chem.238, 235 (1963).
A. Rossmann, D. Kornexl, G. Versini, F. Pichlmayer, G. Lamprecht, La rivista di scienza dell' Alimentazione, accepted for publication.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lamprecht, G., Pichlmayer, F. & Schmid, E.R. Cation-exchange chromatographic isolation of the methionine-bound sulfur in casein hydrolysates for stable-isotope-ratio analysis. Chromatographia 47, 271–277 (1998). https://doi.org/10.1007/BF02466531
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02466531