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Recovery of an alkaline proteinase from fermentation broth using flocculation for cell removal

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Biotechnology Techniques

Summary

Recovery of a bacterial alkaline proteinase after treatment of the fermentation broth with 16 different flocculating additives has been investigated. The polyelectrolyte Sedipur TF 5 was the most effective at 150 ppm and pH 7.0–9.0, giving a 74% yield of enzyme activity.

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Authors and Affiliations

  1. LIKO Research Institute, Miletičova 23, P.O. Box 4, 820 06, Bratislava, Czech & Slovak Federative Republic

    V. Sitkey, M. Minárik & P. Michalík

Authors
  1. V. Sitkey
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  2. M. Minárik
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  3. P. Michalík
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Sitkey, V., Minárik, M. & Michalík, P. Recovery of an alkaline proteinase from fermentation broth using flocculation for cell removal. Biotechnol Tech 6, 49–52 (1992). https://doi.org/10.1007/BF02438687

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  • DOI: https://doi.org/10.1007/BF02438687

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