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Protein content of various regions of rat brain and adult and aging human brain

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Abstract

The protein content of 14 rat brain regions and 44 human brain regions was assayed. With the human tissue we compared brain areas from adult with those from aged subjects. In each case tissue was obtained soon after death and was quickly frozen. Although the heterogeneous distribution of many proteins in the brain is well established, unexpectedly the content of protein per unit weight of fresh tissue showed little variation either regionally or with age. It seems that the various regional heterogeneities of proteins cancel each other, resulting in a fairly homogeneous distribution of the total protein content.

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References

  1. Banay-Schwartz, M., Lajtha, A., and Palkovits, M.: Changes with aging in the in the levels of amino acids in rat CNS structural elements: I. Glutamate and related amino acids. Neurochem. Res., 14: 555–562, 1989.

    Article  PubMed  CAS  Google Scholar 

  2. Banay-Schwartz, M., Lajtha, A., and Palkovits, M.: Changes with aging in the levels of amino acids in rat CNS structural elements: II. Taurine and small neutral amino acids. Neurochem. Res., 14: 563–570, 1989.

    Article  PubMed  CAS  Google Scholar 

  3. Banay-Schwartz, M., Lajtha, A., and Palkovits, M.: Changes with aging in the levels of amino acids in rat CNS structural elements: III. Large neutral amino acids. J. Neurosci. Res., 26:209–216, 1990.

    Article  PubMed  CAS  Google Scholar 

  4. Banay-Schwartz, M., Lajtha, A., and Palkovits, M.: Changes with aging in the levels of amino acids in rat CNS structural elements: IV. Methionine and basic amino acids. J. Neurosci. Res., 26: 217–223, 1990.

    Article  PubMed  CAS  Google Scholar 

  5. Palkovits, M: Isolated removal of hypothalamic or other brain nuclei of the rat. Brain Res., 59: 449–450, 1973.

    Article  PubMed  CAS  Google Scholar 

  6. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265–275, 1951.

    PubMed  CAS  Google Scholar 

  7. Peterson, G.L.: A simplification of the protein assay method of Lowry et al., which is more generally applicable. Anal. Biochem., 83: 346–356, 1977.

    Article  PubMed  CAS  Google Scholar 

  8. Montpied, P., Ginns, E.I., Martin, B.M., Stetler, D., O’Carroll, A.M., Lolait, S.J., Mahan, L.C., and Paul, S.M.: Multiple GABAA receptor alpha subunit mRNAs revealed by developmental and regional expression in rat, chicken, and human brain. FEBS Lett., 258: 94–98, 1989.

    Article  PubMed  CAS  Google Scholar 

  9. Asano, T., Kamiya, N., Morishita, R., and Kato, K.: Immunoassay for the beta gamma subunits of GTP-binding proteins and their regional distribution in bovine brain. J. Biochem., 103: 950–953, 1988.

    PubMed  CAS  Google Scholar 

  10. Mengod, G., Palacios, J.M., Probst, A., and Harris, B.: Regional distribution of the expression of a human stimulatory GTP-binding protein alpha-subunit in the human brain studied by in situ hybridization. Brain Res., 464: 23–29, 1988.

    PubMed  CAS  Google Scholar 

  11. Perlmutter, L.S., Gall, C., Baudry, M., and Lynch, G.: Distribution of calcium-activated protease calpain in the rat brain. J. Comp. Neurol., 296: 269–276, 1990.

    Article  PubMed  CAS  Google Scholar 

  12. Fukuda, T., Adachi, E., Kawashima, S., Yoshiya, I., and Hashimoto, P.H.: Immunohistochemical distribution of calcium-activated neutral proteinases and endogenous CANP inhibitor in the rabbit hippocampus. J. Comp. Neurol., 302: 100–109, 1990.

    Article  PubMed  CAS  Google Scholar 

  13. Kenessey, A., Banay-Schwartz, M., DeGuzman, T., and Lajtha, A.: Increase in cathepsin D activity in rat brain in aging. J. Neurosci. Res., 23: 454–456, 1989.

    Article  PubMed  CAS  Google Scholar 

  14. Kenessey, A., Banay-Schwartz, M., DeGuzman, T., and Lajtha, A.: Regional distribution of brain calpastatin and of calpain II. Activity with casein and with endogenous brain protein substrates. Neurochem. Int., 15: 307–314, 1989.

    Article  CAS  Google Scholar 

  15. Kenessey, A., Banay-Schwartz, M., DeGuzman, T., and Lajtha, A.: Calpain II activity and calpastatin content in brain regions of 3-and 24-month-old rats. Neurochem. Res., 15: 243–249, 1990.

    Article  PubMed  CAS  Google Scholar 

  16. Fages, C., Khelil, M., Rolland, B., Bridoux, A.M., and Tardy, M.: Glutamine synthetase: A marker of an astroglial subpopulation in primary cultures of defined brain areas. Develop. Neurosci., 10: 47–56, 1988.

    CAS  Google Scholar 

  17. Kincaid, R.L., Balaban, C.D., and Billingsley, M.L.: Differential localization of calmodulin-dependent enzymes in rat brain: Evidence for selective expression of cyclic nucleotide phosphodiesterase in specific neurons. Proc. Natl. Acad. Sci. USA, 84: 1118–1122, 1987.

    PubMed  CAS  Google Scholar 

  18. Hirano, A.A., Greengard, P., and Huganir, R.L.: Protein tyrosine kinase activity and its endogenous substrates in rat brain: A subcellular and regional survey. J. Neurochem., 50: 1447–1455, 1988.

    PubMed  CAS  Google Scholar 

  19. Strieder, G., and Sperk, G.: Purification and characterization of prosomatostatin from rat brain. J. Neurochem., 53: 346–353, 1989.

    PubMed  CAS  Google Scholar 

  20. Cosgrove, J.W., Atack, J.R., and Rapoport, S.I.: Regional analysis of rat brain proteins during senescence. Exp. Gerontol., 22: 187–198, 1987.

    Article  PubMed  CAS  Google Scholar 

  21. Zhang, J., Huether, G., and Neuhoff, V.: Regional protein patterns of the embryonic chick brain: Developmental shifts and the effects of light stimulation. Int. J. Dev. Neurosci., 5: 151–165, 1987.

    Article  PubMed  CAS  Google Scholar 

  22. Archives of Gerontology and Geriatrics, Vol. 12, March/June, 1991.

    Google Scholar 

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Authors and Affiliations

  1. Center for Neurochemistry, N.S. Kline Institute for Psychiatric Research, Orangeburg, NY, 10962-2210

    M. Banay-Schwartz, A. Kenessey, T. DeGuzman & A. Lajtha

  2. 1st Department of Anatomy, Semmelweiss University Medical School, Budapest, H-1450, Hungary

    M. Palkovits

Authors
  1. M. Banay-Schwartz
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  2. A. Kenessey
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  3. T. DeGuzman
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  4. A. Lajtha
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  5. M. Palkovits
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Banay-Schwartz, M., Kenessey, A., DeGuzman, T. et al. Protein content of various regions of rat brain and adult and aging human brain. AGE 15, 51–54 (1992). https://doi.org/10.1007/BF02435024

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  • DOI: https://doi.org/10.1007/BF02435024

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