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Pharmacological testing of intracortical interneuronal connections

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Abstract

The present study comprises an attempt to investigate the influence of acetylcholine on the functional connections of the cortical cells and their frequency characteristics. The multineuronal activity was recorded in the sensorimotor cortex of immobilized and freely moving rats, and was subsequently analyzed using the method of cross-correlational analysis. In the first series of experiments, the influence of the neuromediator, acetylcholine (ACh), and calcium chelation, ethyleneglycol tetraacetate (EGTA), on the functional characteristics of adjacent neurons during the iontophoretic application of these substances to cells of the sensorimotor cortex of unanesthetized immobilized rats was investigated. In this case, the inotophoretic application of ACh led to a change in the frequency characteristics of individual neurons, and in the majority of cases did not influence the character of the interneuronal interactions. The application of EGTA led to a decrease in the average frequency of impulse activity for the majority of the neurons, as well as to the disappearance, independent of the running frequency, of excitatory connections in the cross-correlograms, with their subsequent recovery following the termination of exposure to the EGTA. In the second series of experiments, carried out in freely moving rats with a chronically implanted recording electrode, the systemic administration of the acetylcholinesterase blocker, galantamine, led to a substantial intensification of the impulse activity of the cortical neurons, and at the same time did not exert a substantial influence on the network activity of the same neurons. The hypothesis is advanced that the intracortical neuronal interactions may be accomplished independently of extracortical influences that are expressed in the fluctuations of the baseline impulse activity of individual cells. The qualitative assessment that was carried out of the influence of ACh on the functional characteristics of the cortical neurons does not make it possible to identify an influence of ACh (including a modulatory influence) on the formation of intracortical connections. The methods used in this investigation may be used for a further study of the influence of various mediator systems of the brain on the functioning of intracortical interneuronal connections.

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References

  1. P. V. Bukh-Wiener, I. V. Volkov, and G. Kh. Merzhanova, “The spike collector,”Zh. Vyssh. Nerv. Deyat.,40, No. 6, 1194 (1990).

    Google Scholar 

  2. A. G. Galashina and A. V. Bogdanov, “The features of the systemic organization of cortical neurons in various forms of the manifestation of the conditioned reflex to time,”Zh. Vyssh. Nervn. Deyat.,39, No. 3, 469 (1989).

    CAS  Google Scholar 

  3. U. G. Gasanov,The Systemic Activity of Cortical Neurons [in Russian], Nauka, Moscow (1982).

    Google Scholar 

  4. M. N. Zhadin, B. V. Bakharev, and L. P. Yakupova, “A cross-correlational analysis of the baseline activity of cells of the visual cortex that are variously separated in the awake rabbit,”Zh. Vyssh. Nervn. Deyat.,36, No. 3, 529 (1986).

    CAS  Google Scholar 

  5. P. G. Kostyuk,Calcium and Cell Excitability [in Russian], Nauka, Moscow (1989).

    Google Scholar 

  6. B. I. Kotlyar,The Neurobiological Bases of Learning [in Russian], Nauka, Moscow (1989).

    Google Scholar 

  7. R. I. Kruglikov,The Neurochemical Mechanisms of Learning and Memory [in Russian], Nauka, Moscow (1981).

    Google Scholar 

  8. G. Kh. Merzhanova and A. I. Berg, “A statistical analysis of the functional connections between neurons of the visual and motor cortex in various forms of conditioned reflex behavior,”Zh. Vyssh. Nervn. Deyat.,41, No. 2, 291 (1991).

    Google Scholar 

  9. G. L. Gerstain, “Functional association of neurons: detection and interpretation,” in:Neurosciences: Second Study Program, F. O. Schmitt (ed.), Rockefeller Univ. Press, New York (1970), p. 648.

    Google Scholar 

  10. P. A. Kirkwood, “On the use and interpretation of cross-correlation measurements in the mammalian central nervous system,”J. Neurosci. Meth.,1, 107 (1979).

    Article  CAS  Google Scholar 

  11. K. Krnjevic, “Neurotransmitters in cerebral cortex: a general account,” in:Cerebral Cortex: Functional Properties of Cortical Cells, Plenum Press, New York (1984), p. 36.

    Google Scholar 

  12. N. M. Weinberger, J. H. Ashe, R. Metherate, et al., “Returning auditory cortex by learning: a preliminary model of receptive field plasticity,”Concepts in Neurosci.,1, 91 (1990).

    Google Scholar 

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Authors
  1. U. G. Gasanov
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  2. Yu. L. Martinson
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  3. V. N. Khokhlova
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Deceased

Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow. Translated from Zhurnal Vysshei Nervnoi Deyatel'nosti imeni I. P. Pavlova, Vol. 44, No. 6, pp. 1016–1025, November–December, 1994

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Gasanov, U.G., Martinson, Y.L. & Khokhlova, V.N. Pharmacological testing of intracortical interneuronal connections. Neurosci Behav Physiol 26, 37–44 (1996). https://doi.org/10.1007/BF02391154

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  • DOI: https://doi.org/10.1007/BF02391154

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