Summary
This protocol describes the use of the pH-sensitive, intracellularly trapped dye 2′,7′-bis (2-carboxyethyl), 5 (and -6) carboxyfluorescein (BCECF), to characterize the pH regulating mechanisms in cultured human kidney proximal tubule cells. This is a reliable method for intracellular pH measurements and is applicable to single cells, cell suspensions, and confluent cultures.
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This study was supported in part by a Rapid Advisory Group grant from the Veterans Administration, Washington, DC.
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Bergman, J.A., McAteer, J.A., Evan, A.P. et al. Use of the pH-sensitive dye BCECF to study pH regulation in cultured human kidney proximal tubule cells. Journal of Tissue Culture Methods 13, 205–209 (1991). https://doi.org/10.1007/BF02388128
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DOI: https://doi.org/10.1007/BF02388128