Abstract
The principal objective pursued in this study is to establish the base-line data on protein content and on the status of elemental composition in human milk from Sudanese subjects. The protein content was derived by multiplying the nitrogen content by a factor 6.25. The nitrogen was determined using a 14 MeV neutron generator. The median values for crude protein and the total dry matter found in this study were 1.23% (volume) and 104 g/l, respectively. Some minor and trace elements of biological significance namely, Cl, K, Ca, Mn, Fe, Cu, Zn, Cr, Co and Mo were determined using energy dispersive X-ray fluorescence spectrometry. The results obtained show good compatibility with the data reported by the WHO on elemental composition of human milk from different geographical regions.
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References
Elemental Analysis of Biological Materials. Technical Reports Series No. 197, IAEA, Vienna, 1980.
D. Spreadbury, British J. Nutrition, 39 (1978) 601.
B. Sansoni, Instrumentelle Multielementanalyse, Deerfield Beach, FL, VCH, Wienheim, 1985.
R. Jenkins, R. W. Gould, D. Gedcke, Quantitative X-Ray Spectrometry, Marcel Decker, Inc., New York, 1980.
A. S. Ibrahim, Determination of Nitrogen in Foodstuffs Using 14-MeV Neutrons, M.Sc. Thesis, Physics Dept., Khartoum University, Sudan, 1988.
FAO/WHO, Joint Report on Energy and Protein Requirements, FAO Nutrition Meetings Report Series No. 522 and Wld Hlth Org. Tech. Rept. Series, No. 522, 1973.
R. McGill, American Statistician, 32 (1978) 12.
FAO/WHO/UNU, Committee on Energy and Protein Requirements, WHO Technical Reports Series No. 724, Geneva, 1985.
N. F. Butte, C. Garza, E. Obrien, H. Smith, B. L. Nichols, J. Pediatrics, 104 (1984), 95.
WHO/IAEA Joint Report on Minor and Trace Elements in Human Milk, WHO, Geneva, 1989.
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Sam, A.K., Osman, M.M. & El-Khangi, F.A. Determination of protein and trace elements in human milk using NAA and XFR techniques. J Radioanal Nucl Chem 231, 21–23 (1998). https://doi.org/10.1007/BF02387999
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DOI: https://doi.org/10.1007/BF02387999