Summary
Vasoactive intestinal polypeptide is a pancreatic neurotransmitter which augments insulin release. To obtain more detailed information on its mode of action on the pancreatic beta cell we studied the effect of vasoactive intestinal polypeptide on86Rb+ efflux,45Ca2+ uptake, electrical activity and second messenger systems of isolated mouse islets. Vasoactive intestinal polypeptide enhanced insulin release and45Ca2+ uptake in a concentration-dependent manner, and was effective at non-stimulatory and stimulatory glucose levels. It increased glucose-induced electrical activity but was without effect on either glucose-mediated changes of86Rb+ efflux, cAMP or inositol-1,4,5-trisphosphate content. It is suggested that vasoactive intestinal polypeptide augments insulin release by increasing the uptake of Ca2+ into the cell by as yet undefined mechanisms.
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Wahl, M.A., Straub, S.G. & Ammon, H.P.T. Vasoactive intestinal polypeptide-augmented insulin release: actions on ionic fluxes and electrical activity of mouse islets. Diabetologia 36, 920–925 (1993). https://doi.org/10.1007/BF02374473
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DOI: https://doi.org/10.1007/BF02374473