Summary
Polarities of the carotenoids in human serum are very different; many nonpolar carotenoid hydrocarbons (e.g. β-carotene, lycopene) and highly polar hydroxycarotenoids (e. g. β-cryptoxanthin, zeaxanthin, lutein) can be found among them.
Gradient elution chromatography was used for the separation of β-carotene, lycopene, β-cryptoxanthin, lutein and zeaxanthin in serum samples applying amino and cyano packings (Chromsil-NH2, Chromsil-CN). The effects of different stationary phases on the selectivity were compared. The method is particularly suitable for the direct determination of serum levels of carotenoid in serum extracts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
R. Peto, R. Doll, J. D. Buckley, M. B. Sporn, Nature290, 201 (1981).
T. Javor, G. Tóth, J. Szabolcs, M. Bata, J. Morón, G. Mozsik, INT. J. TISS. REAC.V (3), 289 (1983).
Micheline M. Mathews-Roth, Pure Appl. Chem.57, 717 (1985).
M. M. Mathews-Roth, J. N. C. I.69, 279 (1982).
L. Santamaria, A. Bianchi, A. Araboldi, L. Andreoni, P. Bermond, Med. Biol. Environ.10 (1), 359 (1982).
R. Ohmacht, Gy. Tóth, in Chromatography, the State of the Art,H. Kalász andL. S. Ettre (Eds.), Elsevier, Amsterdam, 1985, p. 119.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohmacht, R., Tóth, G. & Voigt, G. HPLC determination of carotenoids in human serum by gradient elution technique. Chromatographia 22, 189–190 (1986). https://doi.org/10.1007/BF02257325
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02257325