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Role of brush border Na+/H+ exchange in canine ileal absorption

  • Intestinal Disorders, Inflammatory Bowel Disease, Immunology, and Microbiology
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Abstract

Na+/H+ exchanger isoforms have been identified in mammalian intestinal enterocytes and cloned: NHE1 on the basolateral membrane regulating intracellular pH; and NHE2 and NHE3 on the brush border serving transcellular absorption of Na+, NHE1 and NHE2 are much more sensitive to inhibition by amiloride than NHE3, theirin vitro IC50s for amiloride being 1 μM, 1 μM and 39 μM, respectively. This study tested the hypothesis that the brush border NHE3 isoform plays the predominant role in basal and meal-stimulated ileal absorption. Absorption studies (N=72) were performed in dogs with 25-cm ileal Thiry-Vella fistulae. Six groups were studied over 4 hr. Perfusion with [14C]PEG and 140 mM Na+ was used to calculate absorption of water, ions, and glucose. Luminal amiloride was administered from the second to the fourth hours at doses of 20 μM in groups 3 and 4 to inhibit NHE1 and NHE2, and 1 mM in groups 5 and 6 to also inhibit NHE3. A 480-kcal canine meal was ingested after the second hour in groups 2, 4, and 6. Meal ingestion was followed by significant increases in water and electrolyte absorption. Amiloride (1 mM) caused significant reductions in basal and meal-stimulated ileal absorption, while the 20 μM dose had no effect on either. These data are consistent with the hypothesis that NHE3, but not NHE2, is involved in basal and meal-stimulated ileal water and Na+ absorption.

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Authors and Affiliations

  1. Departments of Surgery and Medicine, The Johns Hopkins Medical Institutions, 600 N, Wolfe Street, 21287-4606, Baltimore, Maryland

    Michael M. Maher MD, Jacqueline D. Gontarek BA, Ramon E. Jimenez MD, Mark Donowitz MD &  Charles J. Yeo MD

Authors
  1. Michael M. Maher MD
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  2. Jacqueline D. Gontarek BA
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  3. Ramon E. Jimenez MD
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  4. Mark Donowitz MD
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  5. Charles J. Yeo MD
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Additional information

Presented in part at the American College of Surgeons Surgical Forum, Chicago, Illinois, October 1994; and at the Society for Surgery of the Alimentary Tract. San Diego, California, May 1995. Published in abstract form inSurgical Forum 45:187–189, and inGastroenterology 108:A1231, 1995.

Supported in part by National Institutes of Health grant R29-DK41178 (C.J.Y.).

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Maher, M.M., Gontarek, J.D., Jimenez, R.E. et al. Role of brush border Na+/H+ exchange in canine ileal absorption. Digest Dis Sci 41, 651–659 (1996). https://doi.org/10.1007/BF02213119

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  • DOI: https://doi.org/10.1007/BF02213119

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