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Defective interfering particles produced during the replication of measles virus

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Abstract

The RNA species present in purified measles virus preparations after 10−3 diluted passage (DP), three undiluted passages (UP3) and eight undiluted passages (UP8) were analysed by sedimentation on sucrose gradients. The particles contained RNAs sedimenting at 52S and 4S, 52S, 18S and 4S, and 52S, 30S, 18S, 11S and 4S respectively. Measles virus particles produced by UP3 were separated into two homogenous populations by rate zonal centrifugation on sucrose gradients. The rapidly sedimenting population contained encapsidated 52S RNA and was infectious virus. The slowly sedimenting particles contained encapsidated 18S RNA, were non-infectious, and they interfered with the replication of infectious virus.

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Authors and Affiliations

  1. Department of Biochemistry, Queen's University, Belfast

    William W. Hall & Samuel J. Martin

  2. Department of Microbiology and Immunobiology, Queen's University, Belfast

    Ernest Gould

Authors
  1. William W. Hall
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  2. Samuel J. Martin
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  3. Ernest Gould
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Hall, W.W., Martin, S.J. & Gould, E. Defective interfering particles produced during the replication of measles virus. Med Microbiol Immunol 160, 155–164 (1974). https://doi.org/10.1007/BF02121722

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  • DOI: https://doi.org/10.1007/BF02121722

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