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Post-transcriptional regulation of complement C4 in low C4-producing strain of mouse

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Abstract

The expression of the fourth component of complement (C4) of the mouse can differ 20-fold and is determined byC4-high (C4 h) orC4-low (C4 l) alleles. To investigate the molecular mechanisms underlying the differences in C4 expression, we compared the transcriptional activity of theC4 genes between high and low C4-producer strains of mice (B10 and FM vs B10.BR) using nuclear transcriptional and chloramphenicol acetyltransferase (CAT) assays. We also compared the level of C4-specific RNA in total and nuclear RNA of the liver. The results revealed no significant difference in transcriptional activity betweenC4 h andC4 l genes. However, the steady-state levels of C4 mRNA are ten times lower inC4 l strains than inC4 h strains, suggesting that the major regulation of C4 plasma levels occurs at the post-transcriptional level.

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Authors and Affiliations

  1. Department of Immunobiology, Cancer Research Institute, Kanazawa University, 920, Kanazawa, Japan

    Kohzo Nakayama, Sa-nga Pattanakitsakul, Shigeru Yokoyama, Masaru Nonaka & Morinobu Takahashi

  2. Department of Experimental Radiology, Shiga University of Medical Science, Seta, 520-21, Otsu, Shiga, Japan

    Hiroshi Kimura

Authors
  1. Kohzo Nakayama
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  2. Sa-nga Pattanakitsakul
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  3. Shigeru Yokoyama
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  4. Masaru Nonaka
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  5. Morinobu Takahashi
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  6. Hiroshi Kimura
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number D90052.

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Nakayama, K., Pattanakitsakul, Sn., Yokoyama, S. et al. Post-transcriptional regulation of complement C4 in low C4-producing strain of mouse. Immunogenetics 31, 361–367 (1990). https://doi.org/10.1007/BF02115011

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  • DOI: https://doi.org/10.1007/BF02115011

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