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Immunohistochemical location of proteins related to pregnancy-specificβ-glycoprotein (SP1) and placental tissue proteins (PP5, PP8, PP9, PP10, PP11, PP12) in the afterbirth of cynomolgus monkeys (Macaca Fascicularis)

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Zusammenfassung

Mit Hilfe einer Immunglobulin-Enzym-Brücken (PAP)-Technik wurde das Vorkommen und die Lokalisation von Proteinen, die mit dem schwangerschaftsspezifischen β1-Glykoprotein (SP1) und den Plazentagewebsproteinen PP5, PP8, PP9, PP10, PP11 und PP12 antigenverwandt sind, in Plazenten von Cynomolgus-Affen untersucht.

Das Ergebnis der Lokalisation war in Plazenten aus drei verschiedenen Stadien der Schwangerschaft im wesentlichen dasselbe: Der Synzytiotrophoblast (Villi) zeigte eindeutig positive Färbung für SP1, PP8, PP9 und PP11, der Zytotrophoblast (Villi) dagegen nur für PP9. Der Syntheseort für diese Proteine in Plazenten von Cynomolgen sind offensichtlich zur Hauptsache die Synzytiotrophoblastzellen der Villi; PP8 scheint außerdem in den Trophoblastzellen des Chorion gebildet zu werden. PP12 wurde hauptsächlich in Histiozyten gefunden, die reichlich in Dezidua, Nabelschnur, Amnion und Chorion vorkommen. Die Reaktion auf PP5 und PP10 war bei Plazenten von Cynomolgen in allen Schwangerschaftsperioden nur schwach positiv.

Granulozytenähnliche Blutzellen, die gelegentlich im intervillösen Raum der Plazenta vorkommen, zeigten bei Cynomolgen ähnlich wie beim Menschen eine stark positive Reaktion auf PP8, PP10 und PP12.

Insgesamt brachte die Lokalisation der Proteine in Plazenten von Cynomolgen ähnliche Ergebnisse wie die kü rzlich durchgeführten Untersuchungen an Plazenten des Menschen. Cynomolgus-Affen sind daher möglicherweise als Tiermodelle für die Untersuchung der biologischen und klinischen Bedeutung der neuen Plazentaproteine gut geeignet.

Summary

The occurrence and location of proteins antigenically related to human pregnancy specific β1-glycoprotein (SP1) and to human placental tissue proteins (PP5, PP8, PP9, PP10, PP11, PP12) were studied in the afterbirth (placenta, membranes, decidua, and umbilical cord) of cynomolgus monkeys with use of an immunoglobulin enzyme bridge (PAP) technique.

The results were basically the same throughout pregnancy. The syncytiotrophoblast of villi showed clear positive stainings for SP1, PP8, PP9, and PP11, whereas the cytotrophoblast (villi) was found to show clear positive staining for PP9 only. The main sources of proteins related to SP1, PP9, and PP11 were the syncytiotrophoblast cells of the villi and of PP8, the syncytiotrophoblast cells of villi as well as the chorionic trophoblast cells. PP12 was mainly located in histiocytes found in the decidua, umbilical cord, amnion, and chorion. There were only traces of PP5 and PP10 in the placentae of cynomolgus monkeys throughout gestation. As in human placentae some granulocyte-like blood cells in the intervillous space were strongly positive for PP8, PP10, and PP12.

The findings in the afterbirth of cynomolgus monkeys were similar to those in the human; the monkey could thus serve as a model for the investigation of the new placental proteins.

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  1. Research Laboratories, Behringwerke AG, P.O. Box 1140, D-3550, Marburg/Lahn, Federal Republic of Germany

    N. Inaba, T. Renk, E. Weinmann & H. Bohn

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  1. N. Inaba
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  2. T. Renk
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  3. E. Weinmann
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  4. H. Bohn
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Inaba, N., Renk, T., Weinmann, E. et al. Immunohistochemical location of proteins related to pregnancy-specificβ-glycoprotein (SP1) and placental tissue proteins (PP5, PP8, PP9, PP10, PP11, PP12) in the afterbirth of cynomolgus monkeys (Macaca Fascicularis). Arch. Gynecol. 230, 195–204 (1981). https://doi.org/10.1007/BF02111803

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  • DOI: https://doi.org/10.1007/BF02111803

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