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Expression of HLA-DR antigens on colonic epithelium around lymph follicles

An anomalous expression in Crohn's disease

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Abstract

The relationship between the lymph follicle and expression of HLA-DR antigens on the colonic epithelium was investigated. Colonic specimens containing lymph follicles were examined immunohistochemically for HLA-DR expression. Thirty-six biopsy specimens (29 from macroscopically uninvolved areas and seven from aphthoid lesions) were obtained from 10 patients with Crohn's disease, and another 43 specimens were obtained from 33 patients with various intestinal diseases (non-Crohn's disease). HLA-DR antigens were expressed on the epithelium around the lymph follicle in both groups. However, the rate of expression (31/36) in Crohn's disease was significantly higher than that (5/43) in non-Crohn's diseases (P<0.001). In addition, the extent and intensity of expression were broader and stronger in Crohn's disease than in non-Crohn's disease. This study is the first demonstration of anomalous HLA-DR expression on the epithelium around the lymph follicle in Crohn's disease.

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References

  1. Bodmer WF, Albert E, Bodmer JG, Dausset J, Kissmeyer-Nielsen F, Mayr W, Payne R, van Rood JJ, Trnka Z, Walford RL: Nomenclature for factors of the HLA system 1984. Hum Immunol 11:117–125, 1984

    PubMed  Google Scholar 

  2. Ogasawara K, Kojima H, Ikeda H, Ishikawa N, Kasahara M, Fukasawa Y, Natori T, Wakisaka A, Kikuchi Y, Aizawa M, Iwabuchi K, Ogasawara M, Noguchi M, Geng L, Morikawa K, Onoe K: A study on class II antigens involved in the T cell proliferative responses to PPD using cross-reacting monoclonal antibodies in human and murine system. Immunobiology 171:112–114, 1986

    PubMed  Google Scholar 

  3. Iizuka M, Chiba M, Ohta H, Kodama K, Igarashi K, Arakawa H, Masamune O: Expression of HLA-DR antigens on colonic epithelium in ulcerative colitis. Gastroenterol Jpn 22:571–577, 1987

    PubMed  Google Scholar 

  4. Horie Y, Chiba M, Iizuka M, Masamune O: Class II (HLA-DR, -DP, -DQ) antigens on intestinal epithelia in ulcerative colitis, Crohn's disease, colorectal cancer and normal small intestine. Gastroenterol Jpn 25:575–584, 1990

    PubMed  Google Scholar 

  5. Iizuka M, Chiba M, Horie Y, Masamune O, Ohta H: Lymphoid cell subsets in colonic mucosa and HLA-DR antigens on colonic epithelia in colitis excluding ulcerative colitis and Crohn's disease. Gastroenterol Jpn 25:700–707, 1990

    PubMed  Google Scholar 

  6. Horie Y, Chiba M, Iizuka M, Igarashi K, Masamune O: HLA antigens on colorectal adenoma and cancer cells. Tohoku J Exp Med 160:311–322, 1990

    PubMed  Google Scholar 

  7. Horie Y, Chiba M, Iizuka M, Igarashi K, Masamune O: Colonic lymphoid cell subsets and epithelial HLA-DR antigens in familial polyposis coli. Gastroenterol Jpn 24:632–639, 1989

    PubMed  Google Scholar 

  8. Chiba M, Iizuka M, Horie Y, Igarashi K, Masamune O: HLA-DR antigen expression in macroscopically uninvolved areas of intestinal epithelia in Crohn's disease. Gastroenterol Jpn 24:365–372, 1989

    PubMed  Google Scholar 

  9. Chiba M, Iizuka M, Ishii N, Masamune O: Crohn's disease associated with diffuse lymphoid hyperplasia of the large intestine. A possible role of the lymph follicle in HLA-DR antigen expression on epithelium. Intern Med 31:1271–1276, 1992

    PubMed  Google Scholar 

  10. Tada M, Niki H, Hattori S, Yamaguchi K, Katoh S, Kohli Y, Miyaoka T, Kawai K: Utility of dye scattering method for the observation of the process of ulcerative colitis. Gastroenterol Endosc 17:668–675, 1975 (in Japanese with an English abstract)

    Google Scholar 

  11. Kimura M, Miki K, Ichinose M, Hoshino E, Sano J, Kawamura N, Tashiro T, Kakei N, Matsushima M, Yahagi N, Kurokawa K: A new endoscopic finding of inflammatory bowel disease (IBD). Gastroenterology 98:A181, 1990 (abstract)

  12. Chiba M, Iizuka M, Ohtaka M, Kuwabara T, Masamune O, Mukoujima T, Itoh M: Four cases of the red ring sign. Gastrointestinal Endosc 38:728–730, 1992

    Google Scholar 

  13. Yoshikawa K, Mori K: Aphthoid colitis. Stomach Intest 11:793–801, 1976 (in Japanese with an English abstract)

    Google Scholar 

  14. Isobe Y, Chen ST, Nakane PK, Brown WR: Studies on translocation of immunoglobulins across intestinal epithelium. Improvements in the peroxidase-labelled antibody method for application to study of human intestinal mucosa. Acta Histochem Cytochem 10:161–171, 1977

    Google Scholar 

  15. Wang CY, Al-Katib A, Lane CL, Koziner B, Fu SM: Induction of HLA-DC/DS (Leu 10) antigen expression by human precursor B cell lines. J Exp Med 158:1757–1762, 1983

    PubMed  Google Scholar 

  16. Matsumoto K: Studies of monoclonal antibodies specific to human leukocyte differentiation antigens. J Kurume Med Assoc 47:1382–1397, 1984 (in Japanese with an English abstract)

    Google Scholar 

  17. Austin P, Trowsdale J, Rudd C, Bodmer W, Feldmann M, Lamb J: Functional expression of HLA-DP genes transfected into mouse fibroblasts. Nature 313:61–64, 1985

    PubMed  Google Scholar 

  18. Mayer L, Eisenhardt D, Salomon P, Bauer W, Plous R, Piccinini L: Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease. Gastroenterology 100:3–12, 1991

    PubMed  Google Scholar 

  19. Scott H, Solheim BG, Brandtzaeg P, Thorsby E: HLA-DR like antigens in the epithelium of the human small intestine. Scand J Immunol 12:77–82, 1980

    PubMed  Google Scholar 

  20. Selby WS, Janossy G, Mason DY, Jewell DP: Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. Clin Exp Immunol 53:614–618, 1983

    PubMed  Google Scholar 

  21. Spencer JO, Finn T, Isaacson PG: Expression of HLA-DR antigens on epithelium associated with lymphoid tissue in the human gastrointestinal tract. Gut 27:153–157, 1986

    PubMed  Google Scholar 

  22. Russell GJ, Harmatz PR: Major histocompatibility complex class II expression on enterocytes: To present or not to present. Gastroenterology 100:274–276, 1991

    PubMed  Google Scholar 

  23. Pujol-Borrell R, Todd I, Doshi M, Bottazzo GF, Sutton R, Gray D, Adolf GR, Feldmann M: HLA class II induction in human islet cells by interferon-γ plus tumor necrosis factor or lymphotoxin. Nature 326:304–306, 1987

    PubMed  Google Scholar 

  24. Crawford RM, Finbloom DS, Ohara J, Paul WE, Meltzer MS: B cell stimulatory factor-1 (interleukin 4) activates macrophages for increased tumoricidal activity and expression of Ia antigens. J Immunol 139:135–141, 1987

    PubMed  Google Scholar 

  25. Sakai K, Takiguchi M, Mori S, Kobori O, Morioka Y, Inoko H, Sekiguchi M, Kano K: Expression of class II antigens on gastric carcinoma cells and gastric epithelia: Differential expression of DR, DQ, and DP antigens. J Natl Cancer Inst 79:923–932, 1987

    PubMed  Google Scholar 

  26. Ligumsky M, Simon PL, Karmeli F, Rachmilewitz: Role of interleukin 1 in inflammatory bowel disease—enhanced production during active disease. Gut 31:686–689, 1990

    PubMed  Google Scholar 

  27. Mitsuyama K, Sata M, Tanikawa K: Significance of inter-leukin-6 in patients with inflammatory bowel disease. Gastroenterol Jpn 26:20–28, 1991

    PubMed  Google Scholar 

  28. Laufer I, deSa D: Lymphoid follicular pattern: A normal feature of the pediatric colon. Am J Roentgenol 130:51–55, 1978

    Google Scholar 

  29. Watanabe H, Margulis AR, Harter L: The occurrence of lymphoid nodules in the colon of adults. J Clin Gastroenterol 5:535–539, 1983

    PubMed  Google Scholar 

  30. Morson BC: Pathology of Crohn's disease. Clin Gastroenterol 1:265–277, 1972

    Google Scholar 

  31. Uchida J: An ultrastructural study on active uptake and transport of bacteria by microfold cells (M cells) to the lymphoid follicles in the rabbit appendix. J Clin Electron Microsc 20:379–394, 1987

    Google Scholar 

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Authors and Affiliations

  1. First Department of Internal Medicine, Akita University School of Medicine, 1-1-1, Hondo, 010, Akita City, Japan

    Mitsuro Chiba MD, Masahiro Iizuka MD, Yasuo Horie MD, Nobuaki Ishii MD & Osamu Masamune MD

Authors
  1. Mitsuro Chiba MD
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  2. Masahiro Iizuka MD
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  3. Yasuo Horie MD
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  4. Nobuaki Ishii MD
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  5. Osamu Masamune MD
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Additional information

This work was partly supported by grants from the Research Committee for Intractable Intestinal Diseases, Ministry of Welfare, Japan (Member: Mitsuro Chiba), and from the Japanese Foundation for Research and Promotion of Endoscopy (Mitsuro Chiba).

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Chiba, M., Iizuka, M., Horie, Y. et al. Expression of HLA-DR antigens on colonic epithelium around lymph follicles. Digest Dis Sci 39, 83–90 (1994). https://doi.org/10.1007/BF02090065

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  • DOI: https://doi.org/10.1007/BF02090065

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