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Immunogenicity and safety of recombinantHelicobacter pylori urease in a nonhuman primate

  • Esophageal, Gastric And Duodenal Disorders
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Abstract

Groups of squirrel monkeys (Saimiri spp.), predetermined to be free ofHelicobacter infections in the gastric mucosa, were immunized orally with 0.5–4.5 mg ofHelicobacter pylori recombinant urease (rUrease) and 25–500 µg ofEscherichia coli heat-labile enterotoxin (LT) adjuvant. Oral immunization with rUrease resulted in a markedly elevated serum immunoglobulin G (IgG) antibody response with peak levels at 45 days after immunization. No significant gastric inflammation or cytotoxicity was evident in rUrease immunized monkeys as determined by light and electron microscopy. Twenty-five micrograms of LT was a sufficient and safe adjuvant dosage, whereas higher dosages resulted in diarrhea and lethargy. Animals developed a serum IgG antibody response to LT that did not impede the production of anti-rUrease antibody levels. The results of this investigation indicate that rUrease is immunogenic in a nonhuman primate.

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Author information

Authors and Affiliations

  1. Department of Microbiology, From the Greenville Hospital System/Clemson University Biomedical Cooperative, 29634, South Carolina

    Christian T. K. -H. Stadtländer PhD, J. David Gangemi PhD, Supriya S. Khanolkar MS, Christine M. Kitsos MS, Harold E. Farris Jr. DVM, Linda K. Fulton DVM, Joseph E. Hill DVM, PhD, ACVP, F. Kay Huntington MD, ABIM, Cynthia K. Lee PhD & Thomas P. Monath MD, FACP

  2. Research Services, Clemson University, 29634, South Carolina

    Christian T. K. -H. Stadtländer PhD, J. David Gangemi PhD, Supriya S. Khanolkar MS, Christine M. Kitsos MS, Harold E. Farris Jr. DVM, Linda K. Fulton DVM, Joseph E. Hill DVM, PhD, ACVP, F. Kay Huntington MD, ABIM, Cynthia K. Lee PhD & Thomas P. Monath MD, FACP

  3. Livestock Diagnostic Laboratory, Clemson University, 29224, Columbia, South Carolina

    Christian T. K. -H. Stadtländer PhD, J. David Gangemi PhD, Supriya S. Khanolkar MS, Christine M. Kitsos MS, Harold E. Farris Jr. DVM, Linda K. Fulton DVM, Joseph E. Hill DVM, PhD, ACVP, F. Kay Huntington MD, ABIM, Cynthia K. Lee PhD & Thomas P. Monath MD, FACP

  4. Gastroenterology Associates, P.A., Professional Medical Center at St. Francis Hospital, 29604, South Carolina

    Christian T. K. -H. Stadtländer PhD, J. David Gangemi PhD, Supriya S. Khanolkar MS, Christine M. Kitsos MS, Harold E. Farris Jr. DVM, Linda K. Fulton DVM, Joseph E. Hill DVM, PhD, ACVP, F. Kay Huntington MD, ABIM, Cynthia K. Lee PhD & Thomas P. Monath MD, FACP

  5. OraVax, Inc., 02139, Cambridge, Massachusetts

    Christian T. K. -H. Stadtländer PhD, J. David Gangemi PhD, Supriya S. Khanolkar MS, Christine M. Kitsos MS, Harold E. Farris Jr. DVM, Linda K. Fulton DVM, Joseph E. Hill DVM, PhD, ACVP, F. Kay Huntington MD, ABIM, Cynthia K. Lee PhD & Thomas P. Monath MD, FACP

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  1. Christian T. K. -H. Stadtländer PhD
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  2. J. David Gangemi PhD
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This work was funded by OraVax, Inc., Cambridge, Massachusetts.

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Stadtländer, C.T.K.H., Gangemi, J.D., Khanolkar, S.S. et al. Immunogenicity and safety of recombinantHelicobacter pylori urease in a nonhuman primate. Digest Dis Sci 41, 1853–1862 (1996). https://doi.org/10.1007/BF02088757

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  • DOI: https://doi.org/10.1007/BF02088757

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