Abstract
The protective effect of a human strain ofBifidobacterium bifidum (B. bifidum) against murine group A rotavirus (MRV) was examined in the intestines of BALB/c infected mice. In experiments designed to determine whetherB. bifidum mediated MRV shedding during diarrheal disease, pregnant dams (and their expected litters) were randomly assigned to the following groups: (1) mice infected with MRV alone; (2)B. bifidum-treated + MRV-infected mice; (3)B. bifidum-treated controls; and (4) saline control animals. An enzyme-linked immunosorbent assay (ELISA) for the detection of group A rotavirus was used to measure virus protein. The sensitivity of the MRV antigen detector ELISA was determined by serially diluting the rotavirus antigen in test samples. Antigen was detected in dilution ranges of 1:256–1:4096 during the acute phase and 1:16–1:512 in the recovery phase of MRV clinical disease, in the samples tested. Treatment withB. bifidum significantly reduced shedding of MRV antigen (P<0.009) on days 2–10 postinoculation. The reduction in shedding of virus protein corresponded well with delayed onset of acute diarrhea (P<0.02). Closer examination of tissue cross sections under electron microscopy revealed that theB. bifidum-ingested strain adhered to the epithelium of the small intestine. These results suggest that priming the intestine withB. bifidum is effective against experimental MRV challenge and confirmed the potential usefulness of this detector ELISA for studying the kinetics of group A rotavirus infection in animals and humans.
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Duffy, L.C., Zielezny, M.A., Riepenhoff-Talty, M. et al. Reduction of virus shedding byB. bifidum in experimentally induced MRV infection. Digest Dis Sci 39, 2334–2340 (1994). https://doi.org/10.1007/BF02087647
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DOI: https://doi.org/10.1007/BF02087647