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Could in vitro fertilization with a modified sperm preparation technique be an option to micromanipulations?

  • Andrology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose: This study evaluated the efficiency of in vitro fertilization with a modified sperm preparation technique for male infertility, to avoid systematic recourse to ICSI.

Methods: The sperm function stimulants pentoxifylline, 2-deoxyadenosine, and follicular fluid were used during the sperm preparation of IVF trials for two groups of patients: couples with long-standing infertility and previous IVF failures and couples with male-factor infertility scheduled for their first treatment.

Results: Forty-nine attempts were performed in the first group. The overall fertilization rate (percentage of zygotes with two pronuclei 18 hr after sperm insemination) was 30% and the clinical pregnancy rate per transfer was 33%. For the second group, 30 attempts were performed. The fertilization rate was 29.6% and the clinical pregnancy rate was 62.5% per transfer. The total number of recovered motile sperm appears to be a significant parameter: a cutoff value of 0.25 million motile sperm recovered after capacitation treatment has a good prognostic value for choosing between IVF with sperm enhancers and ICSI.

Conclusions: The described modified IVF could constitute an alternative to the systematic use of ICSI.

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Authors and Affiliations

  1. Departement de Procréation Médicalement Assistée, Centre Médico-Chirurgical et Obstétrical, 19 rue Louis Pasteur, BP 120, 67303, Schiltigheim Cedex, France

    Christiane Wittemer, Jeanine Ohl, Karima Bettahar-Lebugle, Laurence Moreau & Pierre Dellenbach

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  1. Christiane Wittemer
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  2. Jeanine Ohl
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  3. Karima Bettahar-Lebugle
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  4. Laurence Moreau
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  5. Pierre Dellenbach
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Wittemer, C., Ohl, J., Bettahar-Lebugle, K. et al. Could in vitro fertilization with a modified sperm preparation technique be an option to micromanipulations?. J Assist Reprod Genet 13, 726–730 (1996). https://doi.org/10.1007/BF02066427

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  • DOI: https://doi.org/10.1007/BF02066427

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