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Effect of radiation and radioprotection on small intestinal function in canines

  • Intestinal Disorders, Inflammatory Bowel Disease, Immunology, And Microbiology
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Abstract

Radiation with doses >7.5 Gy damages the canine intestinal mucosa, and pretreatment with WR2721 reduces this damage. However, the effects of radiation and of WR2721 onin vivo intestinal transport are unclear. Therefore, we determined canine survival, intestinal transport, and mucosal histology following unilateral abdominal irradiation. Isoperistaltic ileostomies were prepared in 23 dogs under general anesthesia and aseptic conditions. After a three-week recovery period, animals were given either placebo or WR2721, 150 mg/kg intravenously, 30 min prior to 10 Gy cobalt-60 abdominal irradiation. Ileal transport and histology were determined in both groups before exposure and one, four, and seven days after irradiation. Seven-day survival was significantly improved by pretreatment with WR2721 (91% vs 33%,P<0.02). On day 4, both mucosal integrity and net intestinal absorption were significantly better (P<0.05) after WR2721 than after placebo. Thus, radiation-induced damage to the ileal mucosa is accompanied by a reduction in net ileal absorption of water and electrolytesin vivo. In addition, pretreatment with WR2721 improves animal survival while reducing ileal damage and improving intestinal absorption.

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Author notes

  1. Jorge L. Herrera MD

    Present address: Division of Gastroenterology, University of South Alabama College of Medicine, Mastin Building Suite 4G, 2451 Fillingim Street, 36617, Mobile, Alabama

  2. James B. Nold DVM, PhD

    Present address: Pathology Department, Hazleton Wisconsin Inc., 3301 Kinsman Boulevard, 53704, Madison, Wisconsin

Authors and Affiliations

  1. From the Armed Forces Radiobiology Research Institute, 20889-5145, Bethesda, Maryland

    Jorge L. Herrera MD, Roy M. Vigneulle PhD, Thomas Gage MD, Thomas J. MacVittie PhD,  James B. Nold DVM, PhD &  Andre Dubois MD, PhD

  2. Walter Reed Army Institute of Research, 20307-5001, Washington DC

    Jorge L. Herrera MD, Roy M. Vigneulle PhD, Thomas Gage MD, Thomas J. MacVittie PhD,  James B. Nold DVM, PhD &  Andre Dubois MD, PhD

  3. Uniformed Services University of the Health Sciences, 20814-4799, Bethesda, Maryland

    Jorge L. Herrera MD, Roy M. Vigneulle PhD, Thomas Gage MD, Thomas J. MacVittie PhD,  James B. Nold DVM, PhD &  Andre Dubois MD, PhD

Authors
  1. Jorge L. Herrera MD
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  2. Roy M. Vigneulle PhD
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  3. Thomas Gage MD
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  4. Thomas J. MacVittie PhD
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  5. James B. Nold DVM, PhD
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  6. Andre Dubois MD, PhD
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Additional information

Supported by the Armed Forces Radiobiology Research Institute, Defense Nuclear Agency, under work units B3161 and 00082. Views presented in this paper are those of the authors; no endorsement by the Defense Nuclear Agency or the Uniformed Services University of the Health Sciences has been given or should be inferred. Research was conducted according to the principles enunciated in the “Guide of the Care and Use of Laboratory Animals” prepared by the Institute of Laboratory Animal Resources, National Research Council.

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Herrera, J.L., Vigneulle, R.M., Gage, T. et al. Effect of radiation and radioprotection on small intestinal function in canines. Digest Dis Sci 40, 211–218 (1995). https://doi.org/10.1007/BF02063968

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  • DOI: https://doi.org/10.1007/BF02063968

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