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Functional alterations of cardiac subcellular structures during energy deficiency in relation to the metabolic state of the heart muscle cell

Funktionelles Verhalten subzellulärer Strukturen des Myokards während Sauerstoffmangel in Relation zum Metabolitstatus der Herzmuskelzelle

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Summary

The functional behaviour of membrane systems of the cardiac cell during oxygen deficiency was analyzed and the alterations were related to the metabolic state of the tissue as an index of injury.

  1. 1.

    The retention function of the cell membrane for proteins. With increasing energy deficiency the cardiac sarcolemma loses its ability to retain macromolecules (myoglobin, enzymes) within the cell. Close correlations exist between protein release and oxygen supply as well as ATP content of the tissue.

  2. 2.

    Function of isolated mitochondria after ischemia. In parallel with a strong impairment of oxidative phosphorylation (decrease of QO 2, RCI values, phosphorylation rates) the Ca++-transporting activity of mitochondria is continuously depressed with decreasing myocardial ATP.

  3. 3.

    Function of isolated sarcoplasmic reticulum after ischemia. With breakdown of high energy phosphates during ischemia rate and extent of Ca++ binding both decrease markedly.

Zusammenfassung

Das funktionelle Verhalten von Membransystemen der Herzmuskelzelle während Sauerstoffmangel wurde untersucht und die Veränderungen in Beziehung gesetzt zum Metabolitstatus des Gewebes als Index für die Zellschädigung.

  1. 1.

    Die Retentionsfunktion der Zellmembran für Proteine. Mit zunehmendem Energiedefizit verliert die Zellmembran die Fähigkeit, Makromoleküle (Myoglobin, Enzyme) innerhalb der Zelle zu retinieren. Zwischen Proteinfreisetzung und O2-Versorgung bzw. Gewebs-ATP bestehen enge Korrelationen.

  2. 2.

    Funktion isolierter Mitochondrien nach Ischämie. Parallel zur Einschränkung der oxidativen Phosphorylierung (Abnahme von QO 2, RCI-Werten, Phosphorylierungsraten) nimmt die Ca++-Transport-Aktivität der Mitochondrien mit Abfall des Gewebs-ATP kontinuierlich ab.

  3. 3.

    Funktion des isolierten sarkoplasmatischen Retikulums nach Ischämie. Mit dem Zerfall der energiereichen Phosphate während Ischämie werden Rate und Ausmaß der Ca++-Bindung an das SR stark eingeschränkt.

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Author notes

  1. P. G. Spieckermann

    Present address: Institute of Physiology I, University of Goettingen, Humboldtallee 7, D-3400, Goettingen

Authors and Affiliations

  1. Institute of Physiology I, University of Goettingen, Humboldtallee 7, D-3400, Goettingen

    M. M. Gebhard, G. G. Göring, H. Kahles, V. A. Mezger, C. J. Preuße & M. Stellwaag

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  1. P. G. Spieckermann
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  2. M. M. Gebhard
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  3. G. G. Göring
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  6. C. J. Preuße
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  7. M. Stellwaag
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Additional information

Paper, presented at the Erwin Riesch Symposium, Tübingen, April 3–7, 1979

With 3 figures

Supported by the Deutsche Forschungsgemeinschaft, SFB 89-Kardiologie Goettingen.

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Spieckermann, P.G., Gebhard, M.M., Göring, G.G. et al. Functional alterations of cardiac subcellular structures during energy deficiency in relation to the metabolic state of the heart muscle cell. Basic Res Cardiol 75, 97–104 (1980). https://doi.org/10.1007/BF02001400

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