Skip to main content
Log in

Ambroxol inhibits interleukin 1 and tumor necrosis factor production in human mononuclear cells

  • Inflammation and Immunomodulation
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

We have studied the effect of Ambroxol on the production of Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF) in human mononuclear cells (MNC). For this purpose MNC were cultured for 24 h in the presence of endotoxin and different doses of Ambroxol. The results indicate that Ambroxol markedly inhibited IL-1 and TNF production at doses of 10–100 μg/ml, without any apparent toxicity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C.A. Dinarello,Interleukin-1. Review Infectious Diseases6, 51–95 (1984).

    Google Scholar 

  2. B. Beutler and A. Cerami,Cachectin and tumour necrosis factor as two sides of the same biological coin. Nature (Lond.)320, 584–588 (1986).

    Article  Google Scholar 

  3. J. A. Schmidt, C. N. Oliver, J. L. Lepe-Zuniga, I. Green and I. Gery,Silica-stimulated monocytes release fibroblast proliferation factors identical to interleukin 1. A potential role for interleukin 1 in the pathogenesis of silicosis. J. clin. Invest.73, 1462–1472 (1984).

    PubMed  Google Scholar 

  4. A. Suwabe, K. Takahashi, S. Yasui, S. Arai and F. Sendo,Bleomycin-stimulated hamster alveolar macrophages release interleukin-1. Amer. J. Path.132, 512–520 (1988).

    PubMed  Google Scholar 

  5. P. T. Piguet, M. A. Collart, G. E. Grau, Y. Kapanci and P. Vassalli,Tumor necrosis factor/cachectin plays a key role in bleomycin-induced pneumopathy and fibrosis. J. exp. Med.170, 655–663 (1989).

    Article  PubMed  Google Scholar 

  6. D.S. Snyder and E. R. Unanue,Corticosteroids inhibit murine macrophage Ia expression and interleukin 1 production. J. Immunol.129, 1803–1805 (1982).

    PubMed  Google Scholar 

  7. B. Beutler, N. Krochin, I. W. Milsark, C. Luedke and A. Cerami,Control of cachectin (tumor necrosis factor) synthesis: Mechanisms of endotoxin resistance. Science232, 977–980 (1986).

    PubMed  Google Scholar 

  8. P. Ghezzi and C. A. Dinarello,IL-1 induces IL-1. III. Specific inhibition of IL-1 production by IFN-γ. J. Immunol.140, 4238–4244 (1988).

    PubMed  Google Scholar 

  9. S. N. Giri, D. M. Hyde and B. J. Marafino Jr.,Ameliorating effect of murine inteferon gamma on bleomycin-induced lung collagen fibrosis in mice. Biochem. Med. Metabolic Biol.36, 194–197 (1986).

    Article  Google Scholar 

  10. J. W. M. van der Meer, S. Endres, G. Lonnemann, J. G. Cannon, T. Ikejima, S. Okusawa, J. A. Gelfand and C. A. Dinarello,Concentrations of immunoreactive human tumor necrosis factor alpha produced by human mononuclear cells in vitro. J. Leukocyte Biol.43, 216–223 (1988).

    PubMed  Google Scholar 

  11. J. M. Chirgwin, A. E. Przybyla, R. J. MacDonald and W. J. Rutter,Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry18, 5294–5299 (1979).

    PubMed  Google Scholar 

  12. P. E. Auron, A. C. Webb, L. J. Rosenwasser, S. F. Mucci, A. Rich, S. M. Wolff and C. A. Dinarello,Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc. nat. Acad. Sci. (Wash.)81, 7907–7911 (1984).

    Google Scholar 

  13. J. A. Kern, R. J. Lamb, J. C. Reed, R. P. Daniele and P. C. Nowell,Dexamethasone inhibition of interleukin 1 beta production by human monocytes. Posttranscriptional mechanisms. J. Clin. Invest.81, 237–244 (1988).

    PubMed  Google Scholar 

  14. P. J. Knudsen, C. A. Dinarello and T. B. Strom,Prostaglandins posttranscriptionally inhibit monocyte expression of interleukin 1 activity by increasing intracellular cyclic adenosine monophosphate. J. Immunol.137, 3189–3194 (1986).

    PubMed  Google Scholar 

  15. C. Donner,ambroxol in preventing chronic bronchitis exacerbations (a double-blind multicenter trial). Respiration46, suppl. 1, 146 (1984).

    Google Scholar 

  16. M. Luisetti, V. Peona, M. Salmona, A. M. Pagnoni, F. Villani, R. Knerich, M. Genghini, L. Abbà and E. Pozzi,Anbroxol and pulmonary toxicity induced by antineoplastic drugs. International Journal Clinical Pharmacology Research6, 129–136 (1986).

    Google Scholar 

  17. R. A. Stockley, J. Shaw and D. Burnett,Effect of ambroxol of neutrophil chemotaxis in vitro. Agents and Actions24, 292–296 (1988).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bianchi, M., Mantovani, A., Erroi, A. et al. Ambroxol inhibits interleukin 1 and tumor necrosis factor production in human mononuclear cells. Agents and Actions 31, 275–279 (1990). https://doi.org/10.1007/BF01997619

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01997619

Keywords

Navigation