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Bioluminescence assay of calcium with a liquid scintillation counter

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Summary

The luminescence produced by Ca aequorin reaction in dilute solutions decays exponentially over a relatively prolonged period of time. The concentration of total Ca and of free Ca in Ca-EGTA buffers could be determined by measuring the decay of luminescence in a liquid scintillation counter. The method is also suitable for determining total Ca concentration in small tissue samples.

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Authors and Affiliations

  1. Leo Research Laboratories, AB Leo, Box 941, S-25109, Helsingborg, Sweden

    C. Oveby, R. Kant & S. Batra

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  1. C. Oveby
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  2. R. Kant
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  3. S. Batra
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Oveby, C., Kant, R. & Batra, S. Bioluminescence assay of calcium with a liquid scintillation counter. Experientia 39, 928–930 (1983). https://doi.org/10.1007/BF01990443

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  • DOI: https://doi.org/10.1007/BF01990443

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