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Intrinsic factor secretion from isolated gastric mucosal cells of rat and man — two different patterns of secretagogue control

  • Histamine and Gastrointestinal Tract
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Abstract

Intrinsic Factor (IF) secretion was studied using isolated gastric mucosal cells from rat and man. In the rat, IF was localized to the chief cells and its secretion responded most efficaciously to carbachol. DbcAMP and hexoprenaline were less powerful, whereas histamine and pentagastrin lacked any effect. In man, IF secretion derived from the parietal cells and was increasingly enhanced by hexoprenaline, pentagastrin, carbachol, histamine and dbcAMP.

In both species, IF secretion differs with respect to its cellular origin and the pattern of secretagogue control: IF release from rat chief cells is due to muscarinic receptor excitation, whereas IF release from human parietal cells responds predominantly to histamine-H2-receptor activation and seems to be mediated by the cAMP system.

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References

  1. R. H. Allen,Human vitamin B 12-transport proteins, Prog. Haematol.9, 57–84 (1975).

    Google Scholar 

  2. IUPAC-IUB Commission on Biochemical Nomenclature,The nomenclature of corrinoids (1973 recommendations), Biochemistry13, 1555–1560 (1974).

    Google Scholar 

  3. R. M. Donaldson, Intrinsic factor and the transport of cobalamin. InPhysiology of the Gastrointestinal Tract, pp. 641–658 (Ed.L. R. Johnson). Raven Press, New York 1981.

    Google Scholar 

  4. A. Lewin, A. M. Cheret, A. Soumarmon andJ. Girodet,Méthode pour l'isolement et le tri des cellules de la muqueuse fundique de rat, Biol. Gastroenterol.7, 139–144 (1974).

    Google Scholar 

  5. A. H. Soll, Physiology of isolated canine parietal cells: receptors and effectors regulating function. InPhysiology of the Gastrointestinal Tract, pp. 673–691 (Ed.L. R. Johnson). Raven Press, New York 1981.

    Google Scholar 

  6. A. Boass andT. H. Wilson,Cellular localization of gastric intrinsic factor in the rat, Am. J. Physiol.206, 783–790 (1963).

    Google Scholar 

  7. J. S. Levine, P. K. Nakane andR. H. Allen,Immunocytochemical localization of human intrinsic factor: the nonstimulated stomach, Gastroenterology79, 493–502 (1980).

    PubMed  Google Scholar 

  8. A. H. Soll,The actions of secretagogues on oxygen uptake by isolated mammalian parietal cells, J. clin. Invest.61, 370–380 (1978).

    PubMed  Google Scholar 

  9. A. Sonnenberg, T. Berglindh, M. J. M. Lewin, J. A. Fischer, G. Sachs andA. Blum, Stimulation of acid secretion in isolated gastric cells. InHormone Receptors in Digestion and Nutrition, pp. 337–348 (EdsG. Rosselin, P. Fromageot andS. Bonfils). Elsevier/North Holland Biomedical Press, Amsterdam 1979.

    Google Scholar 

  10. H. C. Burck,Histologische Technik, pp. 128–130. Thieme, Stuttgart 1969.

    Google Scholar 

  11. Ch. Gottlieb, Kam Seng Lau, R. Wasserman andV. Herbert,Rapid charcoal assay for intrinsic factor (IF),gastric juice unsaturated B 12 binding capacity, antibody to IF and serum unsaturated B 12 binding capacity, Blood25, 875–883 (1965).

    PubMed  Google Scholar 

  12. W. B. Castle,Observations on the etiologic relationship of achylia gastrica to pernicious anaemia, Am. J. med. Sci.178, 748–764 (1929).

    Google Scholar 

  13. C. R. Kapadia andR. M. Donaldson,Macromolecular secretion by isolated gastric mucosa. Fundamental differences in pepsinogen and intrinsic factor secretion, Gastroenterology74, 535–539 (1982).

    Google Scholar 

  14. S. Batzri, J. W. Harmon, M. D. Walker, W. F. Thompson andR. Toles,Secretion of intrinsic factor from dispersed mucosal cells isolated from guinea pig and rabbit stomach, Biochim. Biophys. Acta720, 217–221 (1982).

    Article  PubMed  Google Scholar 

  15. H.-J. Ruoff andM. Becker,Histamine-sensitive adenylate cyclase in human gastric mucosa: cellular localization and interaction by PGE 2,somatostatin and secretin, Agents and Actions12, 174–175 (1982).

    Article  PubMed  Google Scholar 

  16. P. J. Hoedemaker, J. Abels, J. J. Wachters, A. Arends andH. O. Nieweg,Investigations about the site of production of Castle's gastric intrinsic factor, Lab. Invest.13, 1394–1402 (1964).

    PubMed  Google Scholar 

  17. P. J. Hoedemaker, J. Abels, J. J. Wachters andH. O. Nieweg,Further investigations about the site of production of Castle's gastric intrinsic factor, Lab. Invest.15, 1163–1173 (1966).

    PubMed  Google Scholar 

  18. S. T. Callender, The parietal cell and intrinsic factor, InRecent Advances in Gastroenterology, pp. 162–172 (EdsJ. Badenoch andB. N. Brooke). Churchill Livingstone, Edinburgh 1972.

    Google Scholar 

  19. W. Kromer, H. Hannemann, K.-Fr. Sewing, N. Steigemann, W. Vivell andU. Weissenborn,Relative potencies of atropine and pirenzepine in antagonizing cholinergic stimulation of guinea pig parietal cell H + secretion and intestinal motility in vitro, Naunyn-Schmiedebergs Arch. Pharmak.321, R55 (1982).

    Google Scholar 

  20. W. J. Thompson, E. D. Jacobson andG. C. Rosenfeld, A role of cyclic AMP in the gastrointestinal tract: receptor control of hydrogen ion secretion by mammalian gastric mucosa. InHandbook Exp. Pharmacol. 58/II, pp. 651–682 (EdsJ. W. Kebabian andJ. A. Nathanson). Springer Verlag, Berlin, Heidelberg, New York 1982.

    Google Scholar 

  21. W. J. Thompson, L. K. Chang, G. C. Rosenfeld andE. D. Jacobson,Activation of rat gastric mucosal adenyl cyclase by secretory inhibitors, Gastroenterology72, 251–254 (1977).

    PubMed  Google Scholar 

  22. A. H. Soll andA. Wollin,Histamine and cyclic AMP in isolated canine parietal cells, Am. J. Physiol.237, E444-E450 (1979).

    PubMed  Google Scholar 

  23. H.-J. Ruoff, M. Wagner, Ch. Günther andB. Maśliński,Adrenergic stimulation of isolated rat gastric mucosal cells. Effect on adenylate cyclase and 14 C-aminopyrine uptake, Naunyn-Schmiedebergs Arch. Pharmak.320, 175–181 (1982).

    Article  Google Scholar 

  24. S. Ardeman, I. Chanarin andJ. C. Doyle,Studies on secretion of gastric intrinsic factor in man, Br. Med. J.2, 600–603 (1964).

    Google Scholar 

  25. M. H. Vatn, E. Schrumpf andJ. Myren,The effect of carbachol and pentagastrin on the gastric secretion of acid, pepsin and intrinsic factor in man, Scand. J. Gastroenterol.10, 55–58 (1975).

    PubMed  Google Scholar 

  26. E. Fellenius, B. Elander, B. Wallmark, U. Haglund, L. Olbe andH. Hela, Studies on acid secretory mechanism and drug action in isolated gastric glands from man. InHormone Receptors in Digestion and Nutrition, pp. 355–360 (EdsG. Rosselin, P. Froamgeot andS. Bonfils). Elsevier North Holland Biomedical Press, Amsterdam 1979.

    Google Scholar 

  27. A. H. Soll,Secretagogue stimulation of 14 C-aminopyrine accumulation by isolated canine parietal cells, Am. J. Physiol.238, G366-G375 (1980).

    PubMed  Google Scholar 

  28. C. R. Kapadia, D. E. Schafer, R. M. Donaldson andE. R. Ebersole,Evidence for involvement of cyclic nucleotides in intrinsic factor secretion by isolated rabbit gastric mucosa, J. clin. Invest.63, 1044–1049 (1979).

    Google Scholar 

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Authors and Affiliations

  1. Medizinische Poliklinik der Universität Bonn, FRG

    W. Schepp & S. E. Miederer

  2. Pharmakologisches Institut der Universität Tübingen, FRG

    H. -J. Ruoff

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  1. W. Schepp
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  2. S. E. Miederer
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  3. H. -J. Ruoff
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Schepp, W., Miederer, S.E. & Ruoff, H.J. Intrinsic factor secretion from isolated gastric mucosal cells of rat and man — two different patterns of secretagogue control. Agents and Actions 14, 522–528 (1984). https://doi.org/10.1007/BF01973863

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