Summary
Decreasing the glucose concentration from 1.0 mg/ml to 0.25 mg/ml has no detrimental effects on postblastocyst embryo development when either dialyzed fetal calf serum (20%) or bovine serum albumin (4.0 mg/ml) is used to supplement Eagle's Basal Medium (BME). Development is reduced in both serum-and BSA-supplemented BME devoid of glucose in comparison to glucose controls. Serum-supplemented media support better overall development than BSA-supplemented media.
Similar content being viewed by others
References
Y.C. Hsu, Devl Biol.33, 403 (1973).
Y.C. Hsu, J. Baskar, L.C. Stevens and J.E. Rash, J. Embryol. exp. Morph.31, 235 (1974).
A.I. Spindle and R.A. Pedersen, J. exp. Zool.186, 305 (1973).
A. McLaren and H.C. Hensleigh, in: The Early Development of Mammals. Cambridge University Press, London 1975.
B.H. Juurlink and S. Fedoroff, In Vitro13 790 (1977).
J.A. DuBois, Thesis, St. Bonaventure University, New York 1977.
S.B. Atienza-Samols and M.I. Sherman, Devl Biol.66, 220 (1978).
R.J. Wordinger and J.A. Kell, IRCS med. Sci.6, 12 (1978).
R.J. Wordinger and R.L. Brinster, Devl Biol.53, 294 (1976).
R.J. Wordinger and J.A. Kell, Experientia34, 881 (1978).
W.R. Earle, E.L. Schilling, T.H. Stark, N.P. Strauss, M.F. Brown and E. Shelton, J. natl Cancer Inst.4, 165 (1943).
A.H. Gates, in: Methods in Mammalian Embryology, p. 64. W.H. Freeman, San Francisco 1971.
R.L. Ehrmann and G.O. Gey, J. natl Cancer Inst.16, 1375 (1956).
S. Stern and J.D. Biggers, J. exp. Zool.168, 61 (1968).
C.B. Ozias and S. Stern, Biol. Reprod.8, 467 (1973).
R.J. Klebe, J. Cell Physiol.86, 231 (1975).
C.B. Ozias and H.M. Weitlauf, J. exp. Zool.177, 147 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hendryx, J.T., Wordinger, R.J. The effects of decreased glucose concentrations on the in vitro development of the post-blastocyst mouse embryo in a fetal calf serum-or bovine serum albumin-supplemented medium. Experientia 35, 1508–1510 (1979). https://doi.org/10.1007/BF01962814
Issue Date:
DOI: https://doi.org/10.1007/BF01962814