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The effect of insulin on the electrophoretic mobility of rat hepatocytes

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The addition of insulin (10 μU) to a suspension of isolated hepatocytes in Krebs-Ringer bicarbonate solution. causes an increase in the negative electrophoretic mobility of the cells from −1.68 μm sec−1 V−1 cm to 2.26 μm sec−1 V−1 cm. This observation supports the findings by other workers that the binding of insulin to its receptor leads to a marked change in the membrane.

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References

  1. B. Desbuquois and P. Cuatrecasas, A. Rev. Med.24, 233 (1973).

    Article  CAS  Google Scholar 

  2. C. R. Park, O. B. Crofford and T. Kono, J. gen. Physiol.52, 296s (1968).

  3. P. G. LeFevre, in: Metabolic Pathways, vol. 4, p. 385. Ed. L. E. Hokin, Academic Press, New York 1973.

    Google Scholar 

  4. J. Avruch, J. R. Carter and D. B. Martin, Biochim. biophys. Acta288, 27 (1972).

    Article  CAS  PubMed  Google Scholar 

  5. R. Scharff and I. G. Wool, Biochem. J.97, 257 (1965).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. G. Guidotti, A. F. Borghetti, G. C. Gazzola, M. Tramacere and V. Dall'Asta, Biochem. J.160, 281 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. T. Clausen and B. R. Martin, Biochem. J.164, 251 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. C. N. Hales and M. C. Perry, in: Adipose Tissue: Regulation and Metabolic Functions, p. 63. Ed. B. Jeanrenaud and D. Hepp, Academic Press, New York 1970.

    Google Scholar 

  9. R. D. Moore, J. Physiol.232, 23 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. W. A. Gavyrck, R. D. Moore and R. C. Thompson, J. Physiol.252, 43 (1975).

    Article  Google Scholar 

  11. P. M. Beigelman and P. B. Hollander, Proc. Soc. exp. Biol. Med.110, 590 (1962).

    Article  CAS  PubMed  Google Scholar 

  12. K. L. Zierler, Am. J. Physiol.197, 515 (1959).

    Article  CAS  PubMed  Google Scholar 

  13. J. Schlessinger, Y. Shechter, M. C. Willingham and I. Pastan, Proc. natl Acad. Sci. USA75, 2659 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. J. Schlessinger, Y. Shechter, P. Cuatrecasas, M. C. Willingham and I. Pastan, Proc. natl Acad. Sci. USA75, 5353 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. J.-L. Carpentier, A. Perrelet and L. Orci, J. Lipid Res.17, 335 (1976).

    Article  CAS  PubMed  Google Scholar 

  16. E. M. Massa, R. D. Morero and R. N. Farias, Biochem. biophys. Res. Commun.66, 115 (1975).

    Article  CAS  PubMed  Google Scholar 

  17. E. M. de Melian, E. M. Massa, R. D. Morero and R. N. Farias, FEBS Lett.92, 142 (1978).

    Article  Google Scholar 

  18. I. A. Bailey, C. J. Garratt and S. M. Wallace, Biochem. Soc. Trans.6, 302 (1978).

    Article  CAS  PubMed  Google Scholar 

  19. F. Hare and C. Lussan, Biochim. biophys. Acta467, 262 (1977).

    Article  CAS  PubMed  Google Scholar 

  20. L. Chen, R. E. Cale, S. Roth and L. Brand, J. biol. Chem.252, 2163 (1977).

    Article  CAS  PubMed  Google Scholar 

  21. P. Luly, C. Crifo and R. Strom, Experientia35, 1300 (1979).

    Article  CAS  PubMed  Google Scholar 

  22. E. J. Ambrose, Cell Electrophoresis. Churchill, London 1965.

    Google Scholar 

  23. J. N. Mehrishi, Prog. Biophys. molec. Biol.25, 1 (1972).

    Article  CAS  Google Scholar 

  24. P. Cuatrecasas and G. P. E. Tell, Proc. natl Acad. Sci. USA70, 485 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. P. Blume, A. Malley, R. J. Knox and G. V. F. Seaman, Nature271, 378 (1978).

    Article  CAS  PubMed  Google Scholar 

  26. M. N. Berry and D. S. Friend, J. Cell Biol.43, 506 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. H. F. DeLuca and P. P. Cohen, in: Manometric Techniques, p. 131. Ed. W. W. Umbreit, R. H. Burris and J. F. Stauffer, Burgess Publ. Co. Minneapolis 1969.

    Google Scholar 

  28. C. R. Park, S. B. Lewis and J. H. Exton, in: Insulin Action, p. 509 Ed. L. B. Fritz. Academic Press, London 1972.

    Google Scholar 

  29. F. V. Vega and T. Kond, Biochim. biophys. Acta512, 221 (1978).

    Article  CAS  PubMed  Google Scholar 

  30. G. Illiano, G. P. E. Tell, M. I. Siegal and P. Cuatrecasas, Proc. natl Acad. Sci. USA70, 2443 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. C. Mukherjee and R. L. Jungas, Biochem. J.148, 229 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. I. G. Wool and P. Cavicchi, Biochemistry6, 1231 (1967).

    Article  CAS  Google Scholar 

  33. J. M. McDonald, D. E. Bruns and L. Jarett, Proc. natl Acad. Sci USA73, 1542 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. M. M. Billah and R. H. Michell, Biochem. J.182, 661 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. G. de Torrontegui and J. Berthet, Biochim. biophys. Acta116, 477 (1966).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Chelsea College, University of London, Manresa Road, SW3 6LX, London, England

    C. B. Delpech, M. C. Perry & D. T. Plummer

Authors
  1. C. B. Delpech
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  2. M. C. Perry
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  3. D. T. Plummer
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Additional information

Acknowledgment. We wish to thank the Medical Research Council for the provision of the microelectrophoresis apparatus and initial running costs of the project.

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Delpech, C.B., Perry, M.C. & Plummer, D.T. The effect of insulin on the electrophoretic mobility of rat hepatocytes. Experientia 36, 1376–1378 (1980). https://doi.org/10.1007/BF01960106

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