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The influence of incubation temperature on the rate of human lymphocyte proliferation in vitro

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Summary

Using the FPG staining technique we have confirmed that incubation temperature has a marked effect on the proliferation rate of cultured lymphocytes. With 48 h-cultures the proportion of 2nd-cycle cells increases linearly over the temperature range 36–39°C. However the absolute size of the increase exhibits interdonor variability.

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Authors and Affiliations

  1. National Radiological Protection Board, Harwell, OX11 ORQ, Didcot, Oxon, England

    R. J. Purrott, N. Vulpis & D. C. Lloyd

  2. Radiopathology Laboratory, CSN Casaccia, Rome, Italy

    R. J. Purrott, N. Vulpis & D. C. Lloyd

Authors
  1. R. J. Purrott
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  2. N. Vulpis
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  3. D. C. Lloyd
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Additional information

We would like to acknowledge the assistance of the Commission of the European Communities for their provision of a travel grant.

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Purrott, R.J., Vulpis, N. & Lloyd, D.C. The influence of incubation temperature on the rate of human lymphocyte proliferation in vitro. Experientia 37, 407–408 (1981). https://doi.org/10.1007/BF01959890

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