Summary
We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors.
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Bernabei, P.A., Santini, V., Dal Pozzo, O. et al. Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium. Experientia 44, 903–906 (1988). https://doi.org/10.1007/BF01941197
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DOI: https://doi.org/10.1007/BF01941197