Résumé
Des mesures quantitatives et des modèles sur gels d'acrilamide ont permi de conclure que les protéines cytoplasmiques préparées de cette manière ne produisent pas de contamination appréciable.
References
E. W. Johns andS. Forrester, Europ. J. Biochem.8, 547 (1969).
J. A. Wilhelm, A. T. Ansevin, A. W. Johnson andL. S. Hnilica, Biochim. biophys. Acta, in press.
J. A. Wilhelm, M. E. McClure andL. S. Hnilica, in preparation.
J. Chauveau, Y. Moule andC. Rouiller, Expl. Cell Res.,11, 317 (1956).
T. Y. Wang, Meth. Enzym.12A, 417 (1967).
T. C. Spelsberg andL. S. Hnilica, Biochim. biophys. Acta228, 202 (1971).
J. Bonner, G. R. Chlakley, M. Dahmus, D. Fambrough, F. Fujimura, R. C. C. Huang, J. Huberman, R. Jensen, K. Marushige, H. Ohlenbusch, B. Olivera andJ. Widholm, Meth. Enzym.12B, 3 (1968).
O. H. Lowry, N. J. Rosebrough, A. L. Farr andR. J. Randall, J. biol. Chem.193, 265 (1951).
K. Weber andM. J. Osborn, J. biol. Chem.244, 4406 (1969).
J. Bonner, M. E. Dahmus, D. Fambrough, R. C. Huang, K. Marushige andD. Y. H. Tuan, Science159, 47 (1968).
T. C. Spelsberg andL. S. Hnilica, Biochim. biophys. Acta228, 212 (1971).
Author information
Authors and Affiliations
Additional information
Supported by grant No. CA-07746 from the United States Public Health Service and grant No. G-138 from the Robert A. Welch Foundation.
Rights and permissions
About this article
Cite this article
Wilhelm, J.A., Groves, C.M. & Hnilica, L.S. Lack of major cytoplasmic protein contamination of rat liver nuclear chromatin. Experientia 28, 514–516 (1972). https://doi.org/10.1007/BF01931850
Issue Date:
DOI: https://doi.org/10.1007/BF01931850