Abstract
Toxicity quantification is important in environmental monitoring, in the field of natural products, and in chemical ecology. The sensitivity and precision of three commonly used methods detecting toxicity in marine environments were compared, using the toxic marine spongeCrambe crambe as a test organism. The paper disk diffusion method (run with marine bacteria) showed the least sensitivity and did not permit toxicity levels to be quantified. The sea urchin and the MICROTOX® tests showed greater sensitivity, and the latter had the higher precision. The relative performance of these methods is discussed. It is concluded that the MICROTOX® bioassay displays the best characteristics for toxicity quantification.
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References
Hagström, B., and Lönning, S., Pharmac. Toxic.32 (1973) 1.
Janssen, C. R., and Persoone, G., Environ. Toxic. Chem.12 (1993) 711.
Reish, D. L., and Oshida, P. S., Manual of methods in aquatic environment research. Part 10. Short-term statics bioassays. FAO Fish. Tech. Pap.247 (1987) 269.
Faulkner, D. J., Nat. Prod. Rep.7 (1990) 269.
Uriz, M. J., Martín, D., and Rosell, D., Mar. Biol.113 (1992) 287.
Berlinck, R., Bifulco, G., Braeckman, J. C., Bruno, I., Daloze, D., Pem, S., Riccio, R., Spampinato, S., and Speroni, E., in: Proceedings 7th Int. Symp. Marine Natural Products, p. 56. Eds M. Piatelli and L. Minale. Consiglio Nazionale delle Ricerche, Capri, Italy 1992.
Jares-Erijman, E. A., Sakai, R., and Rinehart, K. L., J. org. Chem.56 (1991) 5712.
Burlholder, P. R., and Rüetzler, K., Nature222 (1969) 983.
Amade, P., Charroin, C., Baby, C., and Vaceler, J., Mar. Biol.94 (1987) 271.
Van der Vyver, G., Huysecom, J., Braekman, J. C., and Daloze, D., Vie Milieu40 (1990) 285.
Turon, X., and Becerro, M. A., Mar. Ecol. Prog. Ser.82 (1992) 235.
Becerro, M. A., Uriz, M. J., and Turon, X., Mar. Biol.121 (1994) 301.
Rinehart, K. L. Jr., Gloer, J. B., Wilson, G. R., Hughes, R. G. Jr., Li, L. H., Renis, H. E., and McGovern, J. P., Fedn. Proc.42 (1981) 87.
Maida, M., and Coll, J. C., J. chem. Ecol.19 (1993) 2285.
Paul, J. V., and van Alstyne, K. L., Coral Reefs6 (1988) 263.
Bergquist, P. R., and Bedford, J. J., Mar. Biol.46 (1978) 215.
Thompson, J. E., Walker, R. P., and Faulkner, D. J., Mar. Biol.88 (1985) 11.
Uriz, M. J., Martin, D., Turon, X., Ballesteros, E., Hughes, R., and Acebal, C., Mar. Ecol. Prog. Ser.70 (1991) 175.
Becerro, M. A., Lopez, N. I., Turon, X., and Uriz, M. J., J. expl. mar. Biol. Ecol.179 (1994) 195.
Dinnel, P. A., Link, J. M., and Stober, Q. J., Archs environ. Contam. Toxic.16 (1987) 23.
Martín, D., and Uriz, M. J., J. expl mar. Biol. Ecol.173 (1993) 11.
Vasseur, P., Ferard, J. F., Vial, J., and Larbaigt, G., Envir. Pollution34 (1984) 225.
Reteuna, C., Vasseur, P., and Cabridene, R., Hydrobiologia188/189 (1989) 149.
Ribo, J. M., and Rogers, F., Toxic. Assess5 (1990) 135.
Ribo, J. M., and Kaiser, K. L. E., Toxic. Assess.2, (1987) 305.
Kobayashi, N., Publ. Seto mar. biol. Lab.28 (1971) 376.
Oshia, P. S., Goochey, T. K., and Mearns, A. J., Biological Monitoring of Marine Pollutants, p. 389. Eds F. S. Vernberg, A. Calabrese, F. P. Thurberg and W. B. Vernberg. Academic Press, New York 1986.
Zar, J. H., Biostatistical Analysis, Prentice-Hall, Inc., Englewood-Cliffs, New Jersey 1984.
Cariello, L., Giudici, M. N., and Zanetti, L., Comp. Biochem. Physiol.65 (1980) 37.
Kaiser, K. L. E., and Ribo, J. M., Toxic. Assess.3 (1988) 195.
Blum, D. J. W., and Speece, R. E., Journal J. Wat. Pollut. Control Fed.63 (1991) 198.
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Becerro, M.A., Uriz, M.J. & Turon, X. Measuring toxicity in marine environments: critical appraisal of three commonly used methods. Experientia 51, 414–418 (1995). https://doi.org/10.1007/BF01928907
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DOI: https://doi.org/10.1007/BF01928907