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Calcium antagonists and islet function: VII. Effect of calcium deprivation

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Summary

The role of extracellular Ca2+ in the regulation of islet function is investigated. Decreasing extracellular Ca2+ concentrations cause a dose-related inhibition of glucose-induced insulin release. Whereas the efflux of45Ca from perifused islets is transiently increased on exposure to Ca2+-deprived media, it is unaffected by a partial lowering of the extracellular Ca2+ concentration. Under the latter condition, therefore, the observed reduction in the size of the islets' exchangeable calcium pool(s) appears to be due to reduced Ca2+ entry. The proper effect of glucose on Ca handling by the islets is apparently not affected by a lowering in the extracellular Ca2+ concentration. Nevertheless, in islets exposed to glucose and incubated in Ca2+-deprived media, glucose uptake and oxidation and lactate output are decreased, whereas the islet ATP level is increased, as if extracellular Ca2+ shortage were to affect not only the cellular pool of Ca regulating insulin release, but also energy-consuming processes possibly located at the cell membrane.

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Authors and Affiliations

  1. Laboratory of Experimental Medicine, Brussels University School of Medicine, Boulevard de Waterloo 115, B-1000, Brussels, Belgium

    W. J. Malaisse, J. C. Hutton, A. Sener, J. Levy, A. Herchuelz, G. Devis & G. Somers

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  1. W. J. Malaisse
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  2. J. C. Hutton
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  3. A. Sener
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  4. J. Levy
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  5. A. Herchuelz
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  6. G. Devis
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  7. G. Somers
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Malaisse, W.J., Hutton, J.C., Sener, A. et al. Calcium antagonists and islet function: VII. Effect of calcium deprivation. J. Membrain Biol. 38, 193–208 (1978). https://doi.org/10.1007/BF01871922

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  • DOI: https://doi.org/10.1007/BF01871922

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