Summary
Cell-mediated cytotoxicity (CTX) of meningioma patients towards meningioma cells and fibroblasts was studied in vitro before and after surgery, using the3H-proline microcytotoxicity test. When incubated with allogeneic target cells before surgery, lymphocytes from three out of seven donors showed a specific destruction of meningioma tissue, and two were cytotoxic on both types of targets. Five patients were tested against their own tumour and against skin fibroblasts; this was feasible by keeping their lymphocytes frozen in liquid nitrogen until their target cells grew as suitable monolayers in vitro. Three patients showed a specific cytotoxic response. After excision of the tumour a gradual loss of this reactivity was observed in both allogeneic and autologous systems.
Sera of three patients, whose lymphocytes were not reactive against their own tumour, induced antibody-dependent cellular cytotoxicity (ADCC) towards autologous tumours if normal effector cells were used. This type of response was detectable mainly in postoperative sera, and could not be elicited in autologous lymphocytes. On the contrary, autologous sera inhibited CTX of meningioma effector cells.
The data suggest that meningiomas can induce a complex immunological response in the host, which is dependent on the presence or absence of a large tumour burden.
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Bean, M. A., Pees, H. W., Rosen, G., Oettgen, H. F., Prelabelling target ceils with3H-proline as a method for studying lymphocyte cytotoxicity. Nat, Cancer Inst. Monogr.37 (1973), 41–48.
Bean, M. A., Bloom, B. R., Herberman, R. B., Old, L. J., Oettgen, H. F., Klein, G., Terry, W. D., Cell-mediated cytotoxicity for bladder carcinoma: evaluation of a workshop. Cancer Res.35 (1975), 2902–2913.
Bloom, H. J. G., Peckham, M. J., Richardson, A. E., Alexander, P. A., Payne, P. M., Glioblastoma multiforme: a controlled trial to assess the value of specific active immunotherapy in patients treated by radical surgery and radiotherapy. Brit. J. Cancer27 (1973), 253–267.
Böyum, A., Isolation of mononuclear cells and granulocytes from human blood. Scand. J. clin. Lab. Invest.21, supplement 97 (1968), 77–89.
Cannon, G. B., Bonnard, G. D., Djeu, J., West, W. H., Herberman, R. B., Relationship of human natural lymphocyte-mediated cytotoxicity to cytotoxicity of breast-cancer-derived target cells. Int. J. Cancer19 (1977), 487–497.
Fishman, R. A., Brain edema. New Engl. J. Med.293 (1975), 706–711.
Medawar, P. B., Immunity to homologous grafted skin: III. Fate of skin homografts transplanted to brain, to subcutaneous tissue, and to anterior chamber of eye. Brit. J. exp. Path.29 (1948), 58–69.
Meinig, G., Aulig, A., Wende, S., Reulen, H. J., The effect of dexamethasone and diuretics on peritumoral brain edema: comparative study of tissue water content and computed tomography. In: Dynamics of brain edema, pp. 301–305 (Pappius, H. M., Feindel, W., eds.). Berlin-Heidelberg-New York: Springer. 1976.
O'Toole, C., Perlmann, P., Unsgaard, B., Moberger, G., Edsmyr, F., Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer10 (1972), 77–91.
Pape, G. R., Troye, M., Perlman, P., Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. II. Cytotoxicity to allogeneic or autochthonous tumor cells in tissue culture. J. Immunol.118 (1977), 1925–1930.
Pearson, R. G., In vitro and in vivo investigations on antibody-dependent cellular cytotoxicity. Current Topics Microbiol. Immunol.80 (1978), 65–96.
Pees, H. W., Seidel, B., Cell-mediated immune response of patients with meningiomas defined in vitro by a3H-proline microcytotoxicity test. Clin, exp. Immunol.24 (1976), 310–316.
Pees, H. W., Influence of surgery and dexamethasone on cell-mediated immune response in patients with meningiomas. Brit. J. Cancer35 (1977), 537–545.
Powles, R. L., Balchin, L. A., Smith, C., Grant, C. K., Some properties of cryopreserved acute leukemia cells. Cryobiology10 (1973), 282–289.
Pross, H. F., Baines, M. G., Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. VI. A brief review. Cancer Immunol. Immunother.3 (1977), 75–85.
Saxon, A., Stevens, R. H., Ramer, S. J., Clements, P. J., Yu, D. T. Y., Clucocorticoids administered in vivo inhibit human suppressor T lymphocyte function and diminish B lymphocyte responsiveness in in vitro immunoglobulin synthesis. J. clin. Invest.62 (1978), 922–930.
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Pees, H.W. Cytotoxic immune response of meningioma patients towards allogeneic and autologous tumour cells before and after surgery. Acta neurochir 50, 217–227 (1979). https://doi.org/10.1007/BF01808518
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DOI: https://doi.org/10.1007/BF01808518