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Multiparameter analyses of normal and malignant human plasma cells: CD38++, CD56+, CD54+, cIg+ is the common phenotype of myeloma cells

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Abstract

Plasma cells obtained from bone marrow samples of 45 patients with MM, eight patients with MGUS, eight patients with Waldenström's macroglobulinaemia (WM), one patient with immunocytoma, and 12 controls were characterized by immunophenotyping, estimation of DNA content, and labeling index, as well as by morphological analysis. Plasma cells from 37/45 myeloma and 5/8 MGUS patients expressed CD38 and CD56 (N-CAM) on their surface but were negative for other NK cell-associated antigens such as CD16 (FcγRIII) or CD2. All tumor cells of less-differentiated cell type (WM, immunocytoma) and normal polyclonal plasma cells were negative for CD56. CD56-specific mRNA was demonstrated in myeloma cells by northern blot analysis. Another adhesion molecule, ICAM-1 (CD54), was found on plasma cells from all patients and controls examined. Coexpression of CD19 (1/45), CD20 (9/45), or CD33 (3/45) was rare and CD10 with CD14 was expressed by a small tumor cell subpopulation of only one myeloma patient. The individual pattern of surface marker expression was not associated with a special-type myeloma protein isotype, stage or status of disease, LI or histological classification; however, a correlation between CD56 expression or histological classification and DNA content of the tumor cells was found.

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Authors and Affiliations

  1. Abteilung Immunologie und Transfusionsmedizin, Medizinische Hochschule Hannover, Postfach 6101 80, W-3000, Hannover 61, Federal Republic of Germany

    R. Leo, M. Boeker, D. Peest, R. Hein, J. E. Gessner & H. Deicher

  2. Bone Marrow Diagnostics, Universität München, Federal Republic of Germany

    R. Bartl

  3. Division of Oncology, Hematology, Immunology, St. Johannes Hospital, Duisburg

    J. Seibach

  4. Division of Internal Medicine, Ev. Amalie-Sieveking-Krankenhaus Hamburg, Federal Republic of Germany

    G. Wacker

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  1. R. Leo
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  2. M. Boeker
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  3. D. Peest
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  4. R. Hein
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  5. R. Bartl
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  6. J. E. Gessner
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  7. J. Seibach
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  8. G. Wacker
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  9. H. Deicher
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Additional information

This study was supported by theBundesministerium für Forschung und Technologie der Bundesrepublik Deutschland/ DFVLR (01 ZW 8602/0)

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Leo, R., Boeker, M., Peest, D. et al. Multiparameter analyses of normal and malignant human plasma cells: CD38++, CD56+, CD54+, cIg+ is the common phenotype of myeloma cells. Ann Hematol 64, 132–139 (1992). https://doi.org/10.1007/BF01697400

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  • DOI: https://doi.org/10.1007/BF01697400

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